Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	245,180	C→A	R209L (CGT→CTT)	yafK ←	L,D‑transpeptidase domain‑containing protein YafK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	245,180	0	C	A	100.0%	103.8 / NA	30	R209L (CGT→CTT)	yafK	L,D‑transpeptidase domain‑containing protein YafK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/20);  total (10/20)

CACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGC  >  NC_000913/245110‑245254                                                                       |                                                                           cacaACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCt                                                                   <  2:48504/80‑1 (MQ=255) cacaACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTc                                                                  <  1:48505/81‑1 (MQ=255)    aaCTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCa                                                                 >  2:48560/1‑79 (MQ=255)      cTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAagt                                                             <  1:48506/81‑1 (MQ=255)           tGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCg                                                        >  2:48545/1‑81 (MQ=255)                tAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAACGTAGTCGTAAcc                                                   >  2:48553/1‑81 (MQ=255)                 aaCGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAAcc                                                   <  2:48508/80‑1 (MQ=255)                 aaCGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAAcc                                                   <  1:48507/80‑1 (MQ=255)                 aaCGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAAc                                                    <  2:48509/79‑1 (MQ=255)                   cGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCgg                                                <  1:48510/81‑1 (MQ=255)                    ggCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGc                                               >  1:48548/1‑81 (MQ=255)                      cTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCtt                                             <  2:48511/81‑1 (MQ=255)                             aCCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGtt                                        <  1:48512/79‑1 (MQ=255)                                 cGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTccc                                  <  1:48513/81‑1 (MQ=255)                                  gTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTcc                                   >  1:48563/1‑79 (MQ=255)                                     aCGACCATTGACCACAGAAACGGTTGGTTGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAg                                >  1:48564/1‑79 (MQ=255)                                     aCGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGa                               <  1:48514/80‑1 (MQ=255)                                        aCCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTc                           <  1:48515/81‑1 (MQ=255)                                         ccATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTcc                          <  1:48516/81‑1 (MQ=255)                                             tGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTa                       >  1:48557/1‑80 (MQ=255)                                              gACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTa                       >  1:48536/1‑79 (MQ=255)                                                ccACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGt                   <  1:48518/81‑1 (MQ=255)                                                ccACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGt                   <  1:48517/81‑1 (MQ=255)                                                 cacaGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGtt                  <  2:48519/81‑1 (MQ=255)                                                        aCGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAatat           <  1:48520/81‑1 (MQ=255)                                                          ggTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATAttt         <  1:48521/81‑1 (MQ=255)                                                            ttGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTAt       <  2:48522/81‑1 (MQ=255)                                                               gtggCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgc    <  2:48523/81‑1 (MQ=255)                                                                 ggCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgcgc  >  2:48569/1‑81 (MQ=255)                                                                 ggCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgcgc  >  1:48550/1‑81 (MQ=255)                                                                       |                                                                           CACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGC  >  NC_000913/245110‑245254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑