Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,264,101	A→T	D80V (GAT→GTT)	setB →	sugar exporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,101	0	A	T	100.0%	90.7 / NA	26	D80V (GAT→GTT)	setB	sugar exporter SetB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/17);  total (9/17)

GATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAACTAC  >  NC_000913/2264023‑2264177                                                                               |                                                                             gATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTc                                                                            <  2:385162/81‑1 (MQ=255) gATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTc                                                                            <  2:385161/81‑1 (MQ=255)   ttcttcTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGc                                                                          <  1:385163/81‑1 (MQ=255)     cttcttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCa                                                                         <  1:385164/80‑1 (MQ=255)      ttcttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCaaa                                                                       >  2:385197/1‑81 (MQ=255)        cttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCaa                                                                        <  1:385165/78‑1 (MQ=255)        cttTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCaa                                                                        <  2:385166/78‑1 (MQ=255)              cGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGAt                                                               <  1:385167/81‑1 (MQ=255)               ggCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGAtt                                                              <  1:385168/81‑1 (MQ=255)               ggCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGAtt                                                              >  1:385200/1‑81 (MQ=255)                 cAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGt                                                            >  1:385204/1‑81 (MQ=255)                         tCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTtgct                                                    <  2:385169/81‑1 (MQ=255)                               ggATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGt                                              >  1:385202/1‑81 (MQ=255)                                gATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGtt                                             <  1:385171/81‑1 (MQ=255)                                gATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGtt                                             <  2:385170/81‑1 (MQ=255)                                       gTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGt                                       <  1:385172/80‑1 (MQ=255)                                                   ctcGCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCAc                           <  2:385173/80‑1 (MQ=255)                                                      gCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCAcc                          <  2:385175/78‑1 (MQ=255)                                                      gCCGGGCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCAcc                          <  2:385174/78‑1 (MQ=255)                                                         gggCGCTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCttttt                    >  2:385205/1‑81 (MQ=255)                                                            cgcTCTGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGcc                 <  2:385176/81‑1 (MQ=255)                                                                 tGATAAGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGaa            >  1:385210/1‑81 (MQ=255)                                                                     aaGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCg         >  1:385206/1‑80 (MQ=255)                                                                      aGCGCGGCGTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCa       <  1:385177/81‑1 (MQ=255)                                                                          cggcgTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAacta   >  2:825073/1‑81 (MQ=255)                                                                           ggcgTTCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAactac  >  1:385213/1‑81 (MQ=255)                                                                               |                                                                             GATTCTTCTTTACCGGCAGCGCTGTCATTGGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAACTAC  >  NC_000913/2264023‑2264177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑