Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,072	T→C	F267S (TTT→TCT)	yejE →	putative oligopeptide ABC transporter membrane subunit YejE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,072	0	T	C	100.0%	105.4 / NA	30	F267S (TTT→TCT)	yejE	putative oligopeptide ABC transporter membrane subunit YejE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/12);  total (18/12)

TGGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCAC  >  NC_000913/2275995‑2276149                                                                              |                                                                              tGGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGtctat                                                                            >  1:387206/1‑81 (MQ=255)  ggCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGtctatt                                                                           >  2:387187/1‑81 (MQ=255)  ggCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGtctat                                                                            <  2:387159/80‑1 (MQ=255)          cGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAg                                                                   >  1:387194/1‑81 (MQ=255)           gATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGt                                                                  >  2:387207/1‑81 (MQ=255)           gATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGt                                                                  >  1:387192/1‑81 (MQ=255)           gATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGt                                                                  >  1:387196/1‑81 (MQ=255)           gATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGt                                                                  <  1:387160/81‑1 (MQ=255)             tCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTc                                                                <  1:387161/81‑1 (MQ=255)                cAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGAt                                                             <  1:387162/81‑1 (MQ=255)                   tATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAac                                                          <  2:387163/81‑1 (MQ=255)                       atcCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAaccacc                                                      <  1:387164/81‑1 (MQ=255)                          cTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTg                                                   <  2:387165/81‑1 (MQ=255)                             cGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGAcc                                                <  2:387166/81‑1 (MQ=255)                               tCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACctc                                              <  2:387167/81‑1 (MQ=255)                                 atatGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACctcg                                             <  2:387168/80‑1 (MQ=255)                                    tGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACctcgctc                                          >  2:387212/1‑80 (MQ=255)                                     gTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGa                                        >  2:387202/1‑81 (MQ=255)                                      ttGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGAt                                       <  2:387169/81‑1 (MQ=255)                                         ccTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTc                                    >  1:387203/1‑81 (MQ=255)                                          cTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTcc                                   >  2:387208/1‑81 (MQ=255)                                               gCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGc                              >  1:387199/1‑81 (MQ=255)                                                    ggTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCgg                         >  2:387205/1‑81 (MQ=255)                                                    ggTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCgg                         >  1:387213/1‑81 (MQ=255)                                                       cgcgACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTc                       >  2:387193/1‑80 (MQ=255)                                                          gACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTAcc                   >  1:387201/1‑81 (MQ=255)                                                          gACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTAcc                   >  2:387219/1‑81 (MQ=255)                                                           aCCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGATCTCGCTCGATTTCCTCGGCTTCGGTCTACCg                  >  2:387204/1‑81 (MQ=255)                                                                   cTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGtt           >  2:387211/1‑80 (MQ=255)                                                                   cTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGtt           >  2:387217/1‑80 (MQ=255)                                                                   cTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTc          <  2:387170/81‑1 (MQ=255)                                                                           cGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCAc  <  1:387171/81‑1 (MQ=255)                                                                           cGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCAc  >  2:387220/1‑81 (MQ=255)                                                                              |                                                                              TGGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCAC  >  NC_000913/2275995‑2276149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑