| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,281,428 | A→G | K266R (AAA→AGA) | radD → | putative DNA repair helicase RadD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,281,428 | 0 | A | G | 83.9% | 72.1 / 7.2 | 31 | K266R (AAA→AGA) | radD | putative DNA repair helicase RadD |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (10/16); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01 | |||||||||||
TCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGAT > NC_000913/2281356‑2281501 | tcaGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATtg < 1:388062/81‑1 (MQ=255) caGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATtgt > 2:388112/1‑81 (MQ=255) ttATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTAc < 1:388063/81‑1 (MQ=255) ggAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTActgcc > 2:388104/1‑81 (MQ=255) ggAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTActgcc > 2:388105/1‑81 (MQ=255) tgctgcAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCg < 2:388064/80‑1 (MQ=255) gcAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGAt > 2:388108/1‑81 (MQ=255) cAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGa < 2:388065/79‑1 (MQ=255) cAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGa < 1:388066/79‑1 (MQ=255) cgcgCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATgcag < 1:388067/81‑1 (MQ=255) gcgcAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATgcagc < 1:388068/81‑1 (MQ=255) gcgcAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATgcag > 1:388109/1‑80 (MQ=255) cAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACt > 2:388107/1‑81 (MQ=255) aaaGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTg < 1:388069/81‑1 (MQ=255) aaaGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTg < 1:388070/81‑1 (MQ=255) aaGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGa > 2:388106/1‑81 (MQ=255) ggggTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGAtt < 2:388071/81‑1 (MQ=255) atgatTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGc > 2:388127/1‑81 (MQ=255) atTTTTGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGa < 1:388072/80‑1 (MQ=255) tGCCGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACAcccc > 1:388110/1‑81 (MQ=255) ccGCGACGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACAccccc < 2:388073/80‑1 (MQ=255) gcgACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGgc < 2:388074/81‑1 (MQ=255) gACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGgcgc > 1:388117/1‑81 (MQ=255) cGGTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTg < 2:388075/81‑1 (MQ=255) gTTGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAg > 2:388119/1‑81 (MQ=255) ttGAACACGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAgc < 2:388076/81‑1 (MQ=255) acacGCAAGAGAGATTGTGGGATTACTGCCTACCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGa < 2:388077/81‑1 (MQ=255) cacGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGAt < 2:388078/81‑1 (MQ=255) cacGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGAt < 2:388079/81‑1 (MQ=255) cacGCAAGAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGAt < 1:388080/81‑1 (MQ=255) cacGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGAt > 1:388111/1‑81 (MQ=255) | TCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGAT > NC_000913/2281356‑2281501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |