Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,295,698	C→T	G208D (GGC→GAC)	ccmC ←	heme trafficking system membrane protein CcmC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,295,698	0	C	T	100.0%	111.6 / NA	32	G208D (GGC→GAC)	ccmC	heme trafficking system membrane protein CcmC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

ACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGG  >  NC_000913/2295625‑2295775                                                                          |                                                                              acggCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAAt                                                                        <  1:390655/81‑1 (MQ=255)         tttCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACcag                                                                 <  1:390656/80‑1 (MQ=255)         tttCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACcag                                                                 <  1:390657/80‑1 (MQ=255)           tCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACcagcg                                                               >  1:390692/1‑80 (MQ=255)              aTCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACcagcgcagc                                                           <  1:390659/81‑1 (MQ=255)              aTCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACcagcgcagc                                                           <  2:390658/81‑1 (MQ=255)               tCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACcagcgcagcg                                                          >  1:390698/1‑81 (MQ=255)                    aaaaTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGaa                                                     >  2:390699/1‑81 (MQ=255)                     aaaTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAAc                                                    <  1:390660/81‑1 (MQ=255)                      aaTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACg                                                   <  1:390661/81‑1 (MQ=255)                          aaaTTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCAt                                                <  2:390663/80‑1 (MQ=255)                          aaaTTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATc                                               >  2:390704/1‑81 (MQ=255)                          aaaTTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATc                                               <  2:390662/81‑1 (MQ=255)                               aCGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCg                                           >  1:390710/1‑80 (MQ=255)                                  catccgcatcAGCGTCAGCGTGGGAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATc                                       <  1:390664/81‑1 (MQ=255)                                     ccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGa                                     >  1:390706/1‑80 (MQ=255)                                         atcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACtt                                >  2:390701/1‑81 (MQ=255)                                              cgtcagcgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgctg                           <  1:390665/81‑1 (MQ=255)                                               gtcagcgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTtgctg                           >  1:390708/1‑80 (MQ=255)                                                   gcgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATcc                      <  1:390666/81‑1 (MQ=255)                                                    cgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcg                     <  2:390667/81‑1 (MQ=255)                                                     gtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcgc                    >  2:390702/1‑81 (MQ=255)                                                     gtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcgc                    >  2:390713/1‑81 (MQ=255)                                                       ggCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGt                  >  1:390716/1‑81 (MQ=255)                                                          aGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGa               >  2:390711/1‑81 (MQ=255)                                                           gACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGAt              >  1:390703/1‑81 (MQ=255)                                                           gACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGAt              >  1:390700/1‑81 (MQ=255)                                                               ggAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCt          <  2:390668/81‑1 (MQ=255)                                                                gAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTg         <  2:390669/81‑1 (MQ=255)                                                                    aGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGc     <  1:390670/81‑1 (MQ=255)                                                                      gAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAg   >  1:390688/1‑81 (MQ=255)                                                                       aaGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCTgg  >  1:390705/1‑81 (MQ=255)                                                                          |                                                                              ACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGG  >  NC_000913/2295625‑2295775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑