| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,311,795 | C→T | V319I (GTT→ATT) | ompC ← | outer membrane porin C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,311,795 | 0 | C | T | 93.3% | 41.5 / ‑3.6 | 15 | V319I (GTT→ATT) | ompC | outer membrane porin C |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (6/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGAC > NC_000913/2311719‑2311868 | tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAatatat < 1:393722/81‑1 (MQ=255) tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATAttt > 2:393756/1‑80 (MQ=255) tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTc < 2:393723/81‑1 (MQ=255) ccAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATAttt > 2:393750/1‑79 (MQ=255) cagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGat < 1:393724/81‑1 (MQ=255) ggTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGatat < 1:393725/81‑1 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGATATCtt < 1:393726/80‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGATATCTtc < 1:393727/80‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGATATCTtc < 1:393728/80‑1 (MQ=255) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc > 1:393754/1‑81 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGATATCTTCGTCGTCGTAGc < 1:393729/80‑1 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:393749/1‑81 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:393751/1‑80 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAATATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 2:393760/1‑80 (MQ=255) agtagtAGGTAGCACCAACATCAATATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt < 2:393730/81‑1 (MQ=255) aCCAACATCAATATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 2:393759/1‑81 (MQ=255) aacatcaatatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGgga < 1:393731/81‑1 (MQ=255) acatcaatatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggac < 1:393732/81‑1 (MQ=255) | TCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGAC > NC_000913/2311719‑2311868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |