Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,360,002	C→T	E51K (GAA→AAA)	yfaV ←	putative transporter YfaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,360,002	0	C	T	100.0%	109.1 / NA	32	E51K (GAA→AAA)	yfaV	putative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/13);  total (19/13)

TGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACA  >  NC_000913/2359924‑2360078                                                                               |                                                                             tGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTAt                                                                            >  1:402499/1‑81 (MQ=255)     cATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACt                                                                        <  2:402454/81‑1 (MQ=255)      aTCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTc                                                                       <  2:402456/81‑1 (MQ=255)      aTCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTc                                                                       <  2:402455/81‑1 (MQ=255)                  gCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATc                                                            >  1:402493/1‑80 (MQ=255)                    cGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAAt                                                         <  2:402457/81‑1 (MQ=255)                     ggAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATc                                                        >  2:402490/1‑81 (MQ=255)                        acacCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTgg                                                     <  1:402458/81‑1 (MQ=255)                         cacCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGt                                                    <  2:402459/81‑1 (MQ=255)                          acCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTgg                                                     >  1:402496/1‑79 (MQ=255)                          acCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTa                                                   <  1:402460/81‑1 (MQ=255)                                    gCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTg                                         <  1:402461/81‑1 (MQ=255)                                      atatACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTgg                                        >  2:402546/1‑80 (MQ=255)                                       tataCCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCACTCTGGTAGGTCTGTTTGGc                                       >  1:402510/1‑80 (MQ=255)                                            cacaAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAAcc                                 <  1:402463/81‑1 (MQ=255)                                            cacaAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAAcc                                 <  1:402462/81‑1 (MQ=255)                                               aaaGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGat                              <  2:402464/81‑1 (MQ=255)                                                aaGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGata                             >  2:402489/1‑81 (MQ=255)                                                     aaTGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCg                         >  1:402495/1‑80 (MQ=255)                                                     aaTGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCg                         >  2:402501/1‑80 (MQ=255)                                                       tGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAAc                      >  1:402494/1‑81 (MQ=255)                                                        gCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACg                     >  2:402486/1‑81 (MQ=255)                                                         ccTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACgg                    >  1:402503/1‑81 (MQ=255)                                                          cTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTTGCAAAACCGATATTCGAACGGt                   >  2:402511/1‑81 (MQ=255)                                                          cTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGt                   <  1:402465/81‑1 (MQ=255)                                                            gCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTc                  >  1:402508/1‑80 (MQ=255)                                                            gCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTc                  >  2:402516/1‑80 (MQ=255)                                                            gCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTc                  >  2:402488/1‑80 (MQ=255)                                                                ccAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGaa             <  2:402466/81‑1 (MQ=255)                                                                       aTAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGG‑‑TGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGca    >  2:402500/1‑81 (MQ=255)                                                                          aGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGca    >  2:402497/1‑80 (MQ=255)                                                                           gCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGTACGGTCGAGAAACGCCAGcaca  >  1:736334/1‑81 (MQ=255)                                                                               |                                                                             TGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACA  >  NC_000913/2359924‑2360078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑