Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,374,272	T→C	L244L (TTA→TTG)	menE ←	o‑succinylbenzoate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,374,272	0	T	C	100.0%	122.5 / NA	35	L244L (TTA→TTG)	menE	o‑succinylbenzoate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/13);  total (22/13)

CATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGTAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTG  >  NC_000913/2374196‑2374346                                                                             |                                                                           cATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAaca                                                                        >  1:404796/1‑81 (MQ=255)  aTAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAacac                                                                       >  1:404794/1‑81 (MQ=255)  aTAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGC‑‑CCAAGCAACACCg                                                                     >  1:404774/1‑81 (MQ=255)       cGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCtt                                                                  >  2:404787/1‑81 (MQ=255)        gCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt                                                                 <  2:404755/81‑1 (MQ=255)        gCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt                                                                 >  1:404797/1‑81 (MQ=255)         cAAAAGCAACGAATCGCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt                                                                 >  2:404788/1‑80 (MQ=255)         cAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt                                                                 >  1:404802/1‑80 (MQ=255)         cAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTc                                                                <  2:404756/81‑1 (MQ=255)              gCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAggg                                                            >  1:404792/1‑80 (MQ=255)                 aCGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAAc                                                        <  2:404757/81‑1 (MQ=255)                  cGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACg                                                       >  1:404801/1‑81 (MQ=255)                     aTCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGaa                                                    <  1:404758/81‑1 (MQ=255)                        cccTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTa                                                 >  1:404805/1‑81 (MQ=255)                           tGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACgg                                              >  1:404800/1‑81 (MQ=255)                            gCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGt                                             >  2:404795/1‑81 (MQ=255)                               cgcgcgCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTaa                                          >  1:404759/1‑81 (MQ=255)                                gcgcgcCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAAc                                         <  1:404760/81‑1 (MQ=255)                                    gcCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGc                                     <  2:404761/81‑1 (MQ=255)                                       tGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCaaa                                  >  1:404807/1‑81 (MQ=255)                                         ttCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACATCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACg                                >  2:404806/1‑81 (MQ=255)                                           ccGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGc                               >  1:404776/1‑80 (MQ=255)                                            cGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGcc                              <  2:404762/80‑1 (MQ=255)                                            cGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGc                               >  2:404817/1‑79 (MQ=255)                                             gTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCag                            <  1:404763/81‑1 (MQ=255)                                              tCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCaga                           <  2:404765/81‑1 (MQ=255)                                              tCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCaga                           <  2:404764/81‑1 (MQ=255)                                                        cGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTgg                 >  1:404813/1‑81 (MQ=255)                                                         gggATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTgg                 >  2:404814/1‑80 (MQ=255)                                                          ggATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCa               >  2:404810/1‑81 (MQ=255)                                                            aTAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCAc              >  2:404790/1‑80 (MQ=255)                                                                 cgcgccgcCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGa        <  1:404766/81‑1 (MQ=255)                                                                    gccgccAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGc     <  1:404767/81‑1 (MQ=255)                                                                       gccAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGt   >  2:404803/1‑80 (MQ=255)                                                                       gccAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTg  <  2:404768/81‑1 (MQ=255)                                                                             |                                                                           CATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGTAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTG  >  NC_000913/2374196‑2374346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑