Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,394,103	T→A	Q315L (CAG→CTG)	nuoL ←	NADH:quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,394,103	0	T	A	100.0%	144.6 / NA	41	Q315L (CAG→CTG)	nuoL	NADH:quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/16);  total (25/16)

AAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCCAGCAGCAG  >  NC_000913/2394025‑2394176                                                                               |                                                                          aaGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAgg                                                                         <  1:408186/81‑1 (MQ=255)   gAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCt                                                                       >  2:408218/1‑81 (MQ=255)    aaCGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCt                                                                       >  1:408264/1‑80 (MQ=255)       gcgTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATg                                                                   >  1:408212/1‑81 (MQ=255)         gTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGt                                                                 <  1:408187/81‑1 (MQ=255)                atcaAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGt                                                            >  2:408222/1‑79 (MQ=255)                atcaAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTa                                                           <  1:408188/80‑1 (MQ=255)                   aaGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGc                                                        <  2:408190/80‑1 (MQ=255)                   aaGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGc                                                        <  1:408189/80‑1 (MQ=255)                                 cgcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGAt                                         >  1:408221/1‑81 (MQ=255)                                  gcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATg                                        >  1:408220/1‑81 (MQ=255)                                  gcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATg                                        >  1:408223/1‑81 (MQ=255)                                  gcATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATg                                        >  2:408225/1‑81 (MQ=255)                                   cATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGt                                       <  2:408191/81‑1 (MQ=255)                                   cATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGt                                       <  1:408192/81‑1 (MQ=255)                                    aTCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTc                                      <  2:408193/81‑1 (MQ=255)                                      cccATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCgg                                    >  2:408228/1‑81 (MQ=255)                                       ccATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCgg                                    <  1:408194/80‑1 (MQ=255)                                        cATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTc                                  >  1:408219/1‑81 (MQ=255)                                           gCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTg                                >  1:408226/1‑80 (MQ=255)                                               gCACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTAc                             >  1:408236/1‑79 (MQ=255)                                                cACGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAg                          <  2:408195/81‑1 (MQ=255)                                                 aCGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAgc                         >  2:408252/1‑81 (MQ=255)                                                 aCGCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAgc                         <  1:408196/81‑1 (MQ=255)                                                   gCCAAGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAgcgc                       >  1:408224/1‑81 (MQ=255)                                                      aaGCGCGAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCgg                     >  1:408230/1‑80 (MQ=255)                                                            gAGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAAcc              >  2:408243/1‑81 (MQ=255)                                                             aGGAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCg             >  1:408239/1‑81 (MQ=255)                                                              ggAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGTGGCAAAACCgg            >  2:408238/1‑81 (MQ=255)                                                               gAACATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc           >  1:408233/1‑81 (MQ=255)                                                                aaCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc           >  1:408242/1‑80 (MQ=255)                                                                 aCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc           >  2:408267/1‑79 (MQ=255)                                                                 aCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCca         <  2:408198/81‑1 (MQ=255)                                                                 aCATGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCca         <  2:408197/81‑1 (MQ=255)                                                                   aTGTAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagc       <  2:408199/81‑1 (MQ=255)                                                                      tAGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagc    <  2:408200/81‑1 (MQ=255)                                                                       aGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcag     >  2:408240/1‑79 (MQ=255)                                                                       aGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcag     >  2:408244/1‑79 (MQ=255)                                                                       aGCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcag     >  2:408247/1‑79 (MQ=255)                                                                        gCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagcag  >  2:408202/1‑81 (MQ=255)                                                                        gCCAATCAGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagcag  <  1:408201/81‑1 (MQ=255)                                                                               |                                                                          AAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCCAGCAGCAG  >  NC_000913/2394025‑2394176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑