Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,395,087	G→A	R87C (CGC→TGC)	nuoK ←	NADH:quinone oxidoreductase subunit K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,395,087	0	G	A	100.0%	142.3 / NA	40	R87C (CGC→TGC)	nuoK	NADH:quinone oxidoreductase subunit K
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/19);  total (21/19)

TCAATGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGT  >  NC_000913/2395010‑2395161                                                                              |                                                                           tcaatGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGcaacg                                                                         <  1:408443/81‑1 (MQ=255)    atGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAacgacg                                                                      >  2:408483/1‑81 (MQ=255)     tGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACgg                                                                     <  1:408444/81‑1 (MQ=255)     tGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACgg                                                                     >  2:408481/1‑81 (MQ=255)         aaaaTAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTg                                                                   >  2:408479/1‑79 (MQ=255)         aaaaTAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTg                                                                   >  1:408487/1‑79 (MQ=255)         aaaaTAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTg                                                                   >  1:408475/1‑79 (MQ=255)           aataatGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGt                                                               <  1:408445/81‑1 (MQ=255)               atGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgc                                                            <  1:408446/80‑1 (MQ=255)                     aaGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgcagcag                                                       >  2:408485/1‑79 (MQ=255)                     aaGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgcagcag                                                       >  2:408497/1‑79 (MQ=255)                      aGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTgcagcagca                                                     <  2:408447/80‑1 (MQ=255)                          aaGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc                                                 >  2:408493/1‑80 (MQ=255)                          aaGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc                                                 >  1:408494/1‑80 (MQ=255)                           aGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc                                                 >  1:408477/1‑79 (MQ=255)                           aGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAgcgc                                                 >  1:408488/1‑79 (MQ=255)                            gCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAg                                              <  1:408448/81‑1 (MQ=255)                              aTGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGc                                            >  1:408480/1‑81 (MQ=255)                              aTGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGc                                            >  2:408482/1‑81 (MQ=255)                                 ttCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCg                                          <  2:408449/80‑1 (MQ=255)                                   cATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGAt                                        <  2:408450/80‑1 (MQ=255)                                   cATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATa                                       >  2:408495/1‑81 (MQ=255)                                      ccGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATAccc                                    >  2:408496/1‑79 (MQ=255)                                       cgcgCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTc                                    <  1:408451/80‑1 (MQ=255)                                        gcgcATCTCACTTACTGAATCGATGTTCAGGATCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGc                                  <  2:408452/81‑1 (MQ=255)                                            aTCTCACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCt                              >  1:408491/1‑81 (MQ=255)                                              ctcACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgc                            >  1:408502/1‑81 (MQ=255)                                              ctcACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgc                            >  1:408489/1‑81 (MQ=255)                                                cACTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgccg                          >  2:408503/1‑81 (MQ=255)                                                 aCTTACTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTgcc                           <  2:408453/79‑1 (MQ=255)                                                      cTGAATCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCcgcg                     <  2:408454/80‑1 (MQ=255)                                                           tCGATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCt                >  1:408484/1‑80 (MQ=255)                                                             gATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGAt             <  2:408455/81‑1 (MQ=255)                                                             gATGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGAt             <  2:408456/81‑1 (MQ=255)                                                              aTGTTCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATg            <  2:408457/81‑1 (MQ=255)                                                                 ttCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGc          <  1:408458/80‑1 (MQ=255)                                                                 ttCAGGTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCg         <  1:408459/81‑1 (MQ=255)                                                                      gTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAAt    >  2:408490/1‑81 (MQ=255)                                                                      gTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAAt    <  1:408461/81‑1 (MQ=255)                                                                      gTTCTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAAt    <  1:408460/81‑1 (MQ=255)                                                                         cTGGCAACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGt  <  1:408462/80‑1 (MQ=255)                                                                              |                                                                           TCAATGGCAAAATAATGGTTAAGGCAAGCATGTTCATCCGCGCATCTCACTTACTGAATCGATGTTCAGGTTCTGGCGACGACGGTGAAGTTGCAGCAGCAGCGCAAGGCCGATACTCGCTTCTGCCGCCGCGAGGCTGATGGCGAGAATGT  >  NC_000913/2395010‑2395161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑