Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,399,514	T→C	K218E (AAA→GAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,399,514	0	T	C	100.0%	130.7 / NA	37	K218E (AAA→GAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/21);  total (16/21)

GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGG  >  NC_000913/2399437‑2399585                                                                              |                                                                        gTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACg                                                                      >  1:409490/1‑81 (MQ=255)  tAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACgg                                                                     >  2:409488/1‑81 (MQ=255)        tCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa                                                               >  2:409479/1‑81 (MQ=255)            cGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCt                                                           >  2:409470/1‑81 (MQ=255)            cGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCt                                                           <  1:409427/81‑1 (MQ=255)              gggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCg                                                         >  2:409462/1‑81 (MQ=255)               ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCgg                                                        <  2:409428/81‑1 (MQ=255)               ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCg                                                         >  2:409481/1‑80 (MQ=255)                   gATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTg                                                    >  2:409473/1‑81 (MQ=255)                    aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTg                                                    <  2:409430/80‑1 (MQ=255)                    aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGc                                                   <  1:409429/81‑1 (MQ=255)                    aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGc                                                   >  2:409471/1‑81 (MQ=255)                     tGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCg                                                  <  1:409431/81‑1 (MQ=255)                      gTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGt                                                 >  1:409493/1‑81 (MQ=255)                       ttACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGtt                                                >  2:409466/1‑81 (MQ=255)                            gCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATAGCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTc                                           <  2:409432/81‑1 (MQ=255)                             ccGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCg                                          >  1:409475/1‑81 (MQ=255)                                  ggAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTaaa                                     >  1:409477/1‑81 (MQ=255)                                  ggAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGCTGTAACGCTCGGAGTGCGTTTTGTCGGTaaa                                     >  2:409478/1‑81 (MQ=255)                                   gAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTaaa                                     <  2:409434/80‑1 (MQ=255)                                   gAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAAt                                    <  1:409433/81‑1 (MQ=255)                                           cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGt                            <  2:409435/81‑1 (MQ=255)                                              gCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTc                           <  1:409438/79‑1 (MQ=255)                                              gCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCg                          <  1:409436/80‑1 (MQ=255)                                              gCAGATGCTCGGCACAAACTGCGTATCCCATTCACAGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTc                           <  2:409437/79‑1 (MQ=255)                                                aGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGc                       <  1:409439/81‑1 (MQ=255)                                                     cTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAAtt                  >  1:409485/1‑81 (MQ=255)                                                       cGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc                >  2:409486/1‑81 (MQ=255)                                                        ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc                >  2:409498/1‑80 (MQ=255)                                                        ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc                <  2:409440/80‑1 (MQ=255)                                                         gcgcAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGa              <  2:409441/81‑1 (MQ=255)                                                           gcAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGAcc            <  1:409442/81‑1 (MQ=255)                                                                cTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGtt       <  2:409443/81‑1 (MQ=255)                                                                   cATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAc     <  1:409444/80‑1 (MQ=255)                                                                   cATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAc     <  1:409445/80‑1 (MQ=255)                                                                    atatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCg   <  2:409446/81‑1 (MQ=255)                                                                     tatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCgg  <  1:409447/81‑1 (MQ=255)                                                                              |                                                                        GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGG  >  NC_000913/2399437‑2399585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑