Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,411,277	T→C	M26V (ATG→GTG)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,277	0	T	C	100.0%	78.9 / NA	23	M26V (ATG→GTG)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/9);  total (14/9)

GGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA  >  NC_000913/2411203‑2411351                                                                           |                                                                           ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCAc                                                                      >  2:411558/1‑81 (MQ=255)  gAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCAct                                                                     <  2:411519/81‑1 (MQ=255)   aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCActc                                                                    >  1:411547/1‑81 (MQ=255)     aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTg                                                                  <  1:411520/81‑1 (MQ=255)         cTTCTGTTACTGTCAGCGTTCCGCTTAATTCAAACGCTACAATAACAAACAAATCGACCGCATCCACACTCACTCTTccct                                                              >  2:411563/1‑80 (MQ=255)            cTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGt                                                             <  1:411522/79‑1 (MQ=255)            cTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGt                                                             <  2:411521/79‑1 (MQ=255)              gggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAATCGACCGCATCCACACGCACTCTGCCCGTCGc                                                          >  2:411559/1‑80 (MQ=255)                 aCTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCaaa                                                       >  1:411553/1‑80 (MQ=255)                     tCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAg                                                   <  2:411523/80‑1 (MQ=255)                            tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATgg                                            >  1:411555/1‑80 (MQ=255)                                    aTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCa                                   <  1:411524/81‑1 (MQ=255)                                         aaCGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                              <  2:411525/81‑1 (MQ=255)                                              gACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGa                            >  1:411560/1‑78 (MQ=255)                                                  aTAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                     >  1:411556/1‑81 (MQ=255)                                                       aaacaaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                >  1:411562/1‑81 (MQ=255)                                                         acaaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa              <  1:411526/81‑1 (MQ=255)                                                          caaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa             >  1:411569/1‑81 (MQ=255)                                                          caaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa             >  2:411554/1‑81 (MQ=255)                                                             aGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt           >  1:411552/1‑80 (MQ=255)                                                              gCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa         <  1:411527/81‑1 (MQ=255)                                                                gACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc       >  2:411570/1‑81 (MQ=255)                                                                     cATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca  >  2:411572/1‑81 (MQ=255)                                                                           |                                                                           GGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA  >  NC_000913/2411203‑2411351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑