Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,193	G→T	E191D (GAG→GAT)	yfcG →	disulfide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,193	0	G	T	87.5%	62.3 / 3.4	24	E191D (GAG→GAT)	yfcG	disulfide reductase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (7/14);  total (9/15)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTT  >  NC_000913/2421115‑2421266                                                                               |                                                                          ctgctgGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCg                                                                         >  2:413418/1‑81 (MQ=255)         cGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGtt                                                                 <  2:413381/81‑1 (MQ=255)                 aaTGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGccc                                                          <  1:413382/80‑1 (MQ=255)                  aTGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTg                                                        <  1:413383/81‑1 (MQ=255)                  aTGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTg                                                        >  1:413411/1‑81 (MQ=255)                     ccTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCa                                                     <  1:413384/81‑1 (MQ=255)                     ccTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCa                                                     >  2:413422/1‑81 (MQ=255)                         gACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCgg                                                 <  2:413385/81‑1 (MQ=255)                                 aGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCAc                                         >  2:413416/1‑81 (MQ=255)                                    gAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGc                                      >  1:413414/1‑81 (MQ=255)                                         gACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTaaa                                  <  1:413386/80‑1 (MQ=255)                                         gACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTaa                                   >  2:413417/1‑79 (MQ=255)                                           ccTAGCAATGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAg                                >  2:413426/1‑80 (MQ=255)                                                  aTGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTc                        <  2:413388/81‑1 (MQ=255)                                                  aTGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTc                        <  2:413387/81‑1 (MQ=255)                                                   tGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCg                       <  2:413389/81‑1 (MQ=255)                                                   tGTATCCGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCg                       <  2:413390/81‑1 (MQ=255)                                                        ccGGCAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGtgat                  >  1:413419/1‑81 (MQ=255)                                                            cAGTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGc              <  2:413391/81‑1 (MQ=255)                                                              gTCAAGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGt            >  1:413424/1‑81 (MQ=255)                                                                 aaGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCg         <  2:413392/81‑1 (MQ=255)                                                                  aGAACTGGCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCgg        <  2:413393/81‑1 (MQ=255)                                                                         gCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGtt  <  1:413395/80‑1 (MQ=255)                                                                         gCATGATCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGt   <  1:413394/79‑1 (MQ=255)                                                                               |                                                                          CTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTT  >  NC_000913/2421115‑2421266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑