Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,426,400	G→T	S130* (TCG→TAG)	hisJ ←	histidine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,426,400	0	G	T	100.0%	86.5 / NA	25	S130* (TCG→TAG)	hisJ	histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/16);  total (9/16)

TGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAG  >  NC_000913/2426330‑2426477                                                                       |                                                                              tGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGt                                                                     <  1:414235/81‑1 (MQ=255)  gCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTc                                                                    >  2:414272/1‑81 (MQ=255)   cccAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTc                                                                    <  1:414236/80‑1 (MQ=255)        tGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGa                                                              <  2:414238/81‑1 (MQ=255)        tGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGa                                                              <  2:414237/81‑1 (MQ=255)            cATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGt                                                          >  1:414271/1‑81 (MQ=255)             aTTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTc                                                         <  2:414239/81‑1 (MQ=255)              ttACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCa                                                        <  1:414240/81‑1 (MQ=255)                              gggTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGccacca                                        >  2:414274/1‑81 (MQ=255)                                gtggtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGccaccacc                                      <  2:414241/81‑1 (MQ=255)                                   gtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCaaa                                   <  2:414242/81‑1 (MQ=255)                                      cccTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACga                                <  1:414243/81‑1 (MQ=255)                                           cAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATc                           >  2:414287/1‑81 (MQ=255)                                           cAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATc                           <  2:414244/81‑1 (MQ=255)                                                  ccTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCg                    <  2:414245/81‑1 (MQ=255)                                                   cTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGt                   <  2:414246/81‑1 (MQ=255)                                                    tACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTa                  >  2:414286/1‑81 (MQ=255)                                                        cGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGt              >  1:414289/1‑81 (MQ=255)                                                        cGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGt              >  2:414280/1‑81 (MQ=255)                                                          tttGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGttt            >  1:414247/1‑81 (MQ=255)                                                              ccTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTc         <  2:414248/80‑1 (MQ=255)                                                              ccTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCg        <  1:414249/81‑1 (MQ=255)                                                                  tCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTg     <  2:414250/80‑1 (MQ=255)                                                                   cAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGa    <  1:414251/80‑1 (MQ=255)                                                                    aGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAg  >  1:414281/1‑81 (MQ=255)                                                                       |                                                                              TGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAG  >  NC_000913/2426330‑2426477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑