Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	269,523	G→A	S220S (AGC→AGT)	perR ←	putative transcriptional regulator PerR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	269,523	0	G	A	100.0%	93.9 / NA	27	S220S (AGC→AGT)	perR	putative transcriptional regulator PerR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/9);  total (18/9)

CGATTTCACGTTCAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAGCTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGT  >  NC_000913/269447‑269596                                                                             |                                                                          cGATTTCACGTTCAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTAc                                                                       >  2:53005/1‑81 (MQ=255) cGATTTCACGTTCAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTAc                                                                       >  1:53009/1‑81 (MQ=255)          gTTCAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATc                                                              <  1:52981/81‑1 (MQ=255)           ttCAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATc                                                              >  1:53012/1‑80 (MQ=255)           ttCAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATc                                                              >  1:53025/1‑80 (MQ=255)             cAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCg                                                             >  2:53008/1‑79 (MQ=255)                   gtagtTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCa                                                      >  1:53023/1‑80 (MQ=255)                      gtTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAat                                                  >  2:53016/1‑81 (MQ=255)                            aTTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTgg                                            >  1:53020/1‑81 (MQ=255)                                   cacaACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGCCAAATCGAAGGCCATAATTATTGGGTGGCGt                                     >  2:53021/1‑81 (MQ=255)                                    acaACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGt                                     >  1:53022/1‑80 (MQ=255)                                      aaCTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCAt                                  >  2:53028/1‑81 (MQ=255)                                          cccAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTa                              >  1:53027/1‑81 (MQ=255)                                           ccAAGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCtt                               <  2:52982/79‑1 (MQ=255)                                             aaGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTc                           >  1:53017/1‑81 (MQ=255)                                             aaGCCGTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTc                           <  2:52983/81‑1 (MQ=255)                                                  gTCAACTGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTc                      <  2:52984/81‑1 (MQ=255)                                                        tGCGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCa                >  2:53038/1‑81 (MQ=255)                                                          cGGCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCAt               <  1:52985/80‑1 (MQ=255)                                                           ggCAGCAATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATcc             <  2:52986/81‑1 (MQ=255)                                                             cagcaATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCtt           >  2:53031/1‑81 (MQ=255)                                                                caATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTcc        <  2:52987/81‑1 (MQ=255)                                                                caATCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTc         >  2:53024/1‑80 (MQ=255)                                                                 aaTCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCa       >  1:53026/1‑81 (MQ=255)                                                                  aTCGCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCAc      >  1:53035/1‑81 (MQ=255)                                                                     gCCATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTgg   <  2:52988/81‑1 (MQ=255)                                                                      ccATAAAACTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGt  <  1:52989/81‑1 (MQ=255)                                                                             |                                                                          CGATTTCACGTTCAGCCAGTAGTTTTGATTCAAGCACAACTCCCAAGCCGTCAACTGCGGCAGCAATCGCCATAAAGCTACGGTCAAATCGAAGGCCATAATTATTGGGTGGCGTCATCTTATTCGCCTCAAACCATCCTTTCCACTGGT  >  NC_000913/269447‑269596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑