Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,430,546	G→A	T73I (ACC→ATC)	cvpA ←	colicin V production protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,430,546	0	G	A	100.0%	114.9 / NA	33	T73I (ACC→ATC)	cvpA	colicin V production protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/19);  total (14/19)

CCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGG  >  NC_000913/2430469‑2430623                                                                              |                                                                              cccGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAg                                                                            <  1:414979/81‑1 (MQ=255)  ccGATCGGTGCCTGACAACCCCGTTTTCTCCGCCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGc                                                                           <  2:414980/81‑1 (MQ=255)   cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCg                                                                          <  2:414981/81‑1 (MQ=255)      tCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATa                                                                       <  1:414982/81‑1 (MQ=255)        ggTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATaaa                                                                     >  2:415030/1‑81 (MQ=255)                aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTAc                                                               <  2:414983/79‑1 (MQ=255)                  aaCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACcgc                                                            >  2:415036/1‑80 (MQ=255)                    ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGAt                                                         <  1:414984/81‑1 (MQ=255)                       cGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGc                                                      <  1:414985/81‑1 (MQ=255)                            tctcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATcc                                                 >  1:415040/1‑81 (MQ=255)                                  ccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTc                                           <  1:414986/81‑1 (MQ=255)                                   caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTc                                           >  1:415041/1‑80 (MQ=255)                                   caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg                                          <  1:414987/81‑1 (MQ=255)                                   caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg                                          <  2:414988/81‑1 (MQ=255)                                    aaCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg                                          >  2:415035/1‑80 (MQ=255)                                    aaCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCg                                          >  1:415034/1‑80 (MQ=255)                                        ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAAc                                       >  2:415039/1‑79 (MQ=255)                                            tATCACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTc                                 >  1:415038/1‑81 (MQ=255)                                               cACGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTc                              >  1:415062/1‑81 (MQ=255)                                                aCGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTc                              <  2:414989/80‑1 (MQ=255)                                                 cGAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCtt                            >  1:415037/1‑81 (MQ=255)                                                  gAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCtt                            >  2:415046/1‑80 (MQ=255)                                                  gAAGTTCACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTc                           <  2:414990/81‑1 (MQ=255)                                                        cACGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGcc                     >  2:415032/1‑81 (MQ=255)                                                          cGATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCg                   <  2:414991/81‑1 (MQ=255)                                                           gATAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGt                  <  1:414992/81‑1 (MQ=255)                                                            aTAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTa                 <  1:414994/81‑1 (MQ=255)                                                            aTAGCACCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTa                 <  1:414993/81‑1 (MQ=255)                                                                 aCCAACGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCa            <  2:414995/81‑1 (MQ=255)                                                                     aCGATCAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACt        <  2:414996/81‑1 (MQ=255)                                                                          cAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAg   >  1:415042/1‑81 (MQ=255)                                                                          cAGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAg   >  2:415053/1‑81 (MQ=255)                                                                           aGGATAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAgg  <  1:414997/81‑1 (MQ=255)                                                                              |                                                                              CCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGG  >  NC_000913/2430469‑2430623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑