Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,433,798	A→G	L43L (CTT→CTC)	accD ←	acetyl‑CoA carboxyltransferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,433,798	0	A	G	100.0%	88.5 / NA	26	L43L (CTT→CTC)	accD	acetyl‑CoA carboxyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/16);  total (10/16)

CCTTCATCTAACAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCAAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACACCCCTTCAGGAATGCT  >  NC_000913/2433724‑2433875                                                                           |                                                                              ccttcATCTAACAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGAt                                                                           <  2:415682/79‑1 (MQ=255)     cATCTAACAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGtt                                                                     >  1:415724/1‑81 (MQ=255)      aTCTAACAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGtt                                                                     <  1:415683/80‑1 (MQ=255)      aTCTAACAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTc                                                                    <  1:415684/81‑1 (MQ=255)           aCAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCa                                                                  <  1:415685/78‑1 (MQ=255)           aCAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGc                                                                <  1:415688/80‑1 (MQ=255)           aCAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGc                                                                <  2:415687/80‑1 (MQ=255)           aCAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCt                                                               <  2:415686/81‑1 (MQ=255)               gCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAgc                                                           <  1:415689/81‑1 (MQ=255)                 tATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAgcgc                                                         >  2:415733/1‑81 (MQ=255)                  aTGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGcgcg                                                        >  2:415726/1‑81 (MQ=255)                             tACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCt                                             >  2:415716/1‑81 (MQ=255)                              aCGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCt                                             >  2:415719/1‑80 (MQ=255)                              aCGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCt                                             >  2:415720/1‑80 (MQ=255)                                   cTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGc                                       <  1:415690/81‑1 (MQ=255)                                      tCATACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAg                                     <  2:415691/80‑1 (MQ=255)                                         tACGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTAt                                 <  1:415692/81‑1 (MQ=255)                                          aCGCATGTGATGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTAt                                 >  1:415732/1‑80 (MQ=255)                                                   aTGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAg                        <  2:415693/80‑1 (MQ=255)                                                   aTGGTCACACTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGt                       >  2:415728/1‑81 (MQ=255)                                                          cacTTCGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACAcc                >  2:415727/1‑81 (MQ=255)                                                               cGGACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACACCCCt             <  2:415694/79‑1 (MQ=255)                                                                ggACAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACACCCCTTc           <  2:415696/80‑1 (MQ=255)                                                                ggACAGACCTCGAGATTACGTTCCAGCTCAACGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACACCCCTTc           <  2:415695/80‑1 (MQ=255)                                                                  aCAGACCTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACACCCCTTCAg         >  2:415723/1‑80 (MQ=255)                                                                        cTCGAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACACCCCTTCAGGAATGCt  <  2:415697/81‑1 (MQ=255)                                                                           |                                                                              CCTTCATCTAACAGGCTATGCAGGCGATTACGCGCTGTCATACGCATGTGATGGTCACACTTCGGACAGACCTCAAGATTACGTTCCAGCTCAGCGCGGTATAAAACCTGACCGCAGCTATCACACTTAGTCCACACCCCTTCAGGAATGCT  >  NC_000913/2433724‑2433875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑