Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,436,213	T→A	D146V (GAT→GTT)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,213	0	T	A	100.0%	108.3 / NA	31	D146V (GAT→GTT)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/16);  total (15/16)

GCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTA  >  NC_000913/2436138‑2436289                                                                            |                                                                             gCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGAtt                                                                         <  2:416117/81‑1 (MQ=255)  cATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGAtt                                                                         >  2:416167/1‑80 (MQ=255)      gACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACgg                                                                    <  1:416119/81‑1 (MQ=255)      gACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACgg                                                                    >  1:416154/1‑81 (MQ=255)            ttttttGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAg                                                              >  2:416156/1‑81 (MQ=255)                       gggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcag                                                     >  1:416158/1‑79 (MQ=255)                       gggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcag                                                     >  1:416162/1‑79 (MQ=255)                       gggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcag                                                     >  1:416170/1‑79 (MQ=255)                        ggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcagc                                                    <  2:416120/79‑1 (MQ=255)                           ggAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAgctg                                               <  2:416121/81‑1 (MQ=255)                               gCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAgctggctg                                           <  2:416122/81‑1 (MQ=255)                                cTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGctggctgg                                          <  2:416123/81‑1 (MQ=255)                                 tGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGt                                         >  1:416163/1‑81 (MQ=255)                                   aaaTCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTc                                        <  1:416124/80‑1 (MQ=255)                                    aaTCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGt                                         >  2:416178/1‑78 (MQ=255)                                            tGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTg                              >  1:416177/1‑81 (MQ=255)                                            tGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTg                              >  2:416174/1‑81 (MQ=255)                                             ggTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTgg                             >  2:416157/1‑81 (MQ=255)                                                aaCGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTAc                          >  1:416168/1‑81 (MQ=255)                                                aaCGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTAc                          >  1:416153/1‑81 (MQ=255)                                                 aCGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACg                         <  2:416125/81‑1 (MQ=255)                                                   gCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGc                       <  1:416126/81‑1 (MQ=255)                                                    cTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCg                      <  2:416127/81‑1 (MQ=255)                                                     tGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGa                     >  2:416191/1‑81 (MQ=255)                                                       aTACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCTATg                   <  2:416128/81‑1 (MQ=255)                                                       aTACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATg                   <  2:416129/81‑1 (MQ=255)                                                           gTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAt               >  1:416165/1‑81 (MQ=255)                                                              aTAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttcc            <  2:416130/81‑1 (MQ=255)                                                                  aCCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccggtt        <  2:416131/81‑1 (MQ=255)                                                                   ccGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccggttc       <  1:416132/81‑1 (MQ=255)                                                                        cTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTa  <  1:416133/81‑1 (MQ=255)                                                                            |                                                                             GCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTA  >  NC_000913/2436138‑2436289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑