Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,460,346	T→A	K42* (AAG→TAG)	fadI ←	3‑ketoacyl‑CoA thiolase FadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,460,346	0	T	A	100.0%	85.8 / NA	25	K42* (AAG→TAG)	fadI	3‑ketoacyl‑CoA thiolase FadI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/15);  total (10/15)

CGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTTCCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAAC  >  NC_000913/2460268‑2460418                                                                               |                                                                         cGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTAcc                                                                        <  1:420481/81‑1 (MQ=255)   aCCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTAccc                                                                       >  2:420514/1‑80 (MQ=255)       gACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAAt                                                                  >  2:420519/1‑81 (MQ=255)        aCCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAAt                                                                  >  2:420515/1‑80 (MQ=255)            aaGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAAcc                                                             <  2:420482/81‑1 (MQ=255)             aGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACcg                                                            <  2:420483/81‑1 (MQ=255)               aCCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACcgcg                                                          >  1:420526/1‑81 (MQ=255)                        tCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCa                                                 >  1:420524/1‑81 (MQ=255)                          aaTCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATg                                               <  2:420484/81‑1 (MQ=255)                                  cggcggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCg                                       >  1:420523/1‑81 (MQ=255)                                   ggcggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGt                                      <  2:420485/81‑1 (MQ=255)                                    gcggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTc                                     <  1:420486/81‑1 (MQ=255)                                     cggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCg                                    >  1:420520/1‑81 (MQ=255)                                        ggATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCt                                 <  1:420487/81‑1 (MQ=255)                                               gCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGa                               <  1:420488/76‑1 (MQ=255)                                                   cGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCaaaa                      <  2:701880/81‑1 (MQ=255)                                                      gCCAGCAGTTCGCCTACCACCAACTACCCTGCATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGc                   <  1:420497/81‑1 (MQ=255)                                                       ccAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcg                  <  2:420489/81‑1 (MQ=255)                                                        cagcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgt                 <  1:420490/81‑1 (MQ=255)                                                          gcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgtac               <  1:420491/81‑1 (MQ=255)                                                            agTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgtacg              >  1:420527/1‑80 (MQ=255)                                                                 gCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAc         <  2:420492/80‑1 (MQ=255)                                                                 gCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAc         <  1:420493/80‑1 (MQ=255)                                                                   cTACCTCCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGc      >  1:420539/1‑81 (MQ=255)                                                                        accaTCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAAc  >  1:420530/1‑80 (MQ=255)                                                                               |                                                                         CGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTTCCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAAC  >  NC_000913/2460268‑2460418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑