Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,490,344	G→A	T536I (ACC→ATC)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,490,344	0	G	A	100.0%	46.8 / NA	15	T536I (ACC→ATC)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

TTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTT  >  NC_000913/2490274‑2490408                                                                       |                                                                 ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTgc                                                         <  1:424242/80‑1 (MQ=255)  tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTgcgc                                                       >  1:424267/1‑81 (MQ=255)   ttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTgcgc                                                       <  2:424243/80‑1 (MQ=255)   ttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTgcgc                                                       >  2:424265/1‑80 (MQ=255)    tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTGcgcg                                                      <  2:424244/80‑1 (MQ=255)      ggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTGCGCGACt                                                   >  1:424264/1‑81 (MQ=255)               tGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCgg                                          >  1:424263/1‑81 (MQ=255)                           cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCAt                              >  2:424269/1‑81 (MQ=255)                                  aGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAg                       >  2:424280/1‑81 (MQ=255)                                    ttCCTGCTGCAGGGTCGATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGtt                     >  2:424274/1‑81 (MQ=255)                                         gctgcAGGGTCGATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCt                >  2:424271/1‑81 (MQ=255)                                                ggTCGATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtgg         <  2:424245/81‑1 (MQ=255)                                                    gATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGa      <  1:424246/80‑1 (MQ=255)                                                    gATGACCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAc     >  2:424273/1‑81 (MQ=255)                                                        aCCACATTAATAATGATCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGtt  <  2:424247/80‑1 (MQ=255)                                                                       |                                                                 TTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTT  >  NC_000913/2490274‑2490408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑