Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,510,615	T→A	V329E (GTG→GAG)	yfeO →	putative transport protein YfeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,510,615	0	T	A	100.0%	88.8 / NA	27	V329E (GTG→GAG)	yfeO	putative transport protein YfeO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/12);  total (15/12)

GCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTG  >  NC_000913/2510538‑2510690                                                                              |                                                                            gCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGtt                                                                          >  1:427582/1‑81 (MQ=255)  cTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGttt                                                                         <  1:427555/81‑1 (MQ=255)          aaTTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGt                                                                 >  2:427600/1‑81 (MQ=255)           aTTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGt                                                                 <  2:427556/80‑1 (MQ=255)           aTTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTg                                                                <  2:427557/81‑1 (MQ=255)                aCTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCAtt                                                           <  2:427558/81‑1 (MQ=255)                  ttGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAg                                                         >  2:427603/1‑81 (MQ=255)                    gccgccCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAggg                                                       >  2:427614/1‑81 (MQ=255)                    gccgccCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAggg                                                       >  2:427608/1‑81 (MQ=255)                    gccgccCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAg                                                         <  1:427559/79‑1 (MQ=255)                       gccCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGtt                                                     >  2:427602/1‑80 (MQ=255)                           tGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGAtgc                                                >  1:427610/1‑81 (MQ=255)                           tGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCACTAGGGTTGAtgc                                                >  2:427599/1‑81 (MQ=255)                                   gctgcCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGa                                         <  1:427560/80‑1 (MQ=255)                                   gctgcCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAg                                        >  1:427606/1‑81 (MQ=255)                                    ctgcCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGc                                       <  2:427561/81‑1 (MQ=255)                                       ccgccAGTGGCTTTCGCGGTGGTCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACg                                    >  2:427617/1‑81 (MQ=255)                                       ccgccAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCAc                                     <  2:427562/80‑1 (MQ=255)                                        cgccAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGt                                   >  1:427612/1‑81 (MQ=255)                                                gCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCccgcc                            <  1:427564/80‑1 (MQ=255)                                                gCTTTCGCGGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCccgcc                            <  1:427563/80‑1 (MQ=255)                                                      gcgGTGGGCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAg                     >  2:427611/1‑81 (MQ=255)                                                            ggCGAATCTTCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCa                <  2:427565/80‑1 (MQ=255)                                                                 aTCTCCCCAGCAGAATTTCCCGGCGTGGCATTAGGGTTGTGGCTGCAGGAGCAAGTAACCGCCGTACCAGCGGCAATAAcc          <  2:427574/81‑1 (MQ=255)                                                                    ttCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGtt       >  2:427595/1‑81 (MQ=255)                                                                    ttCCCGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGtt       >  2:427621/1‑81 (MQ=255)                                                                         ggCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTg  >  2:427622/1‑81 (MQ=255)                                                                              |                                                                            GCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTG  >  NC_000913/2510538‑2510690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑