Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,513,581	C→T	S180S (TCC→TCT)	nupC →	nucleoside:H(+) symporter NupC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,513,581	0	C	T	100.0%	102.9 / NA	30	S180S (TCC→TCT)	nupC	nucleoside:H(+) symporter NupC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/11);  total (19/11)

AAGATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCCATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCACC  >  NC_000913/2513505‑2513656                                                                             |                                                                            aaGATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCg                                                                         >  1:428138/1‑81 (MQ=255)  aGATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGt                                                                        >  2:428154/1‑81 (MQ=255)   gATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGtt                                                                       <  1:428107/81‑1 (MQ=255)    atatCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTg                                                                      >  1:428158/1‑81 (MQ=255)       tCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGt                                                                    <  1:428108/80‑1 (MQ=255)        ccTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTg                                                                   >  2:428145/1‑80 (MQ=255)         cTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCa                                                                 <  2:428109/81‑1 (MQ=255)           cGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCTACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATa                                                               >  2:428155/1‑81 (MQ=255)                aaaTCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGa                                                          >  2:428156/1‑81 (MQ=255)                       ccGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCt                                                   <  2:428110/81‑1 (MQ=255)                         gTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTg                                                  >  2:428140/1‑80 (MQ=255)                         gTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTg                                                  >  2:428143/1‑80 (MQ=255)                             tCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAAcc                                             <  1:428111/81‑1 (MQ=255)                              cGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAAcc                                             >  2:428151/1‑80 (MQ=255)                               gTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCg                                            <  1:428112/80‑1 (MQ=255)                               gTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCg                                            <  2:428113/80‑1 (MQ=255)                                    tACGCCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATAc                                      <  2:428114/81‑1 (MQ=255)                                      cacCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATAcgt                                    >  2:428152/1‑81 (MQ=255)                                         cATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATAcgtcgt                                 >  2:428144/1‑81 (MQ=255)                                             gCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTtgct                             <  1:428115/81‑1 (MQ=255)                                               tGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTtgctgc                           >  2:428150/1‑81 (MQ=255)                                                  cACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCt                        <  2:428116/81‑1 (MQ=255)                                                  cACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCt                        <  1:428117/81‑1 (MQ=255)                                                  cACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCt                        >  2:428157/1‑81 (MQ=255)                                                                  gtgtCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGtt         >  2:428142/1‑80 (MQ=255)                                                                  gtgtCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGtt         >  1:428153/1‑80 (MQ=255)                                                                   tgtCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGtt         >  1:428159/1‑79 (MQ=255)                                                                    gtCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAg      >  2:428167/1‑81 (MQ=255)                                                                      cGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCa    >  1:428149/1‑81 (MQ=255)                                                                        aTGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCAcc  >  1:428148/1‑81 (MQ=255)                                                                             |                                                                            AAGATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCCATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCACC  >  NC_000913/2513505‑2513656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑