Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,519,022	G→A	M70I (ATG→ATA)	yfeD →	putative DNA‑binding transcriptional regulator YfeD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,519,022	0	G	A	100.0%	58.4 / NA	18	M70I (ATG→ATA)	yfeD	putative DNA‑binding transcriptional regulator YfeD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/12);  total (6/12)

CCAGACTTATTCTGGTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAA  >  NC_000913/2518949‑2519089                                                                          |                                                                    ccAGACTTATTCTGGTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATg                                                               >  2:429067/1‑80 (MQ=255)          ttCTGGTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTg                                                        >  1:429065/1‑78 (MQ=255)               gTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAagaga                                                  <  2:429039/79‑1 (MQ=255)                  gATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAAtt                                             >  2:429073/1‑81 (MQ=255)                   aTACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTg                                            >  2:429071/1‑81 (MQ=255)                      cGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTg                                         <  1:429040/81‑1 (MQ=255)                      cGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTg                                         <  2:429041/81‑1 (MQ=255)                              tGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGc                                  <  1:429042/80‑1 (MQ=255)                                cGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTcc                               <  2:429043/81‑1 (MQ=255)                                       aTTCAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGc                         <  1:429044/80‑1 (MQ=255)                                          cAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCAcg                      <  2:429045/80‑1 (MQ=255)                                          cAGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCAcg                      <  2:429046/80‑1 (MQ=255)                                           aGAACACGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCAcgcg                    >  2:429069/1‑81 (MQ=255)                                               cacGCCAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGcccc                <  1:429047/81‑1 (MQ=255)                                                    cAGCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGt           <  2:429048/81‑1 (MQ=255)                                                      gCCTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTAt         <  2:429049/81‑1 (MQ=255)                                                       ccTTCCATAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATc        <  1:429050/81‑1 (MQ=255)                                                             aTAACACACCAATATTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACaaa  >  1:429072/1‑81 (MQ=255)                                                                          |                                                                    CCAGACTTATTCTGGTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAA  >  NC_000913/2518949‑2519089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑