Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,565,882	G→A	P335S (CCG→TCG)	eutA ←	reactivating factor for ethanolamine ammonia‑lyase EutBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,565,882	0	G	A	100.0%	108.3 / NA	31	P335S (CCG→TCG)	eutA	reactivating factor for ethanolamine ammonia‑lyase EutBC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/18);  total (13/18)

CTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGGCAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGGCGC  >  NC_000913/2565805‑2565959                                                                              |                                                                              cTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAg                                                                            >  1:437538/1‑81 (MQ=255)    aaTCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTg                                                                         <  1:437501/81‑1 (MQ=255)    aaTCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTg                                                                         >  2:437541/1‑81 (MQ=255)       cAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTAc                                                                      >  1:437550/1‑81 (MQ=255)        aGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACg                                                                     <  2:437502/81‑1 (MQ=255)          cgcCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGcc                                                                   <  2:437503/81‑1 (MQ=255)               gTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTc                                                                >  2:437549/1‑79 (MQ=255)                 tGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGc                                                            >  2:437542/1‑81 (MQ=255)                     aGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGc                                                            >  1:437545/1‑77 (MQ=255)                      ggCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAt                                                       <  1:437504/81‑1 (MQ=255)                      ggCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAt                                                       >  2:437546/1‑81 (MQ=255)                      ggCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAt                                                       <  1:437505/81‑1 (MQ=255)                       gCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGAtt                                                      <  1:437506/81‑1 (MQ=255)                         ccccACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATtgt                                                    <  1:437507/81‑1 (MQ=255)                           ccaccaGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgc                                                  >  2:437543/1‑81 (MQ=255)                           ccaccaGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgc                                                  <  1:437508/81‑1 (MQ=255)                             accaGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgctg                                                <  1:437509/81‑1 (MQ=255)                                 gATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGtgctgc                                               >  1:437548/1‑78 (MQ=255)                                           atcaATCGGGATCGCCACCGGCAAATTGCGCGGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAg                                  <  2:437511/81‑1 (MQ=255)                                           atcaATCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAg                                  <  2:437510/81‑1 (MQ=255)                                              aaTCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGt                               <  1:437512/81‑1 (MQ=255)                                               aTCGGGATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTa                              <  1:437513/81‑1 (MQ=255)                                                   ggATCGCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATgcg                          <  2:437514/81‑1 (MQ=255)                                                        gCCACCGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGcgcgccc                     <  2:437515/81‑1 (MQ=255)                                                             cGGCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGcgcgcccgcgcc                <  2:437516/81‑1 (MQ=255)                                                              ggCAAATTGCGCAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGcgcgcccgcgcc                >  2:437547/1‑80 (MQ=255)                                                                       cgcAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGt       <  1:437517/80‑1 (MQ=255)                                                                        gcAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg     <  2:437518/81‑1 (MQ=255)                                                                        gcAGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg     >  2:437558/1‑81 (MQ=255)                                                                          aGCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGgcg   >  2:437556/1‑81 (MQ=255)                                                                           gCGACAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGgcgc  >  1:437551/1‑81 (MQ=255)                                                                              |                                                                              CTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGGCAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGGCGC  >  NC_000913/2565805‑2565959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑