Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,571,342	T→G	N137T (AAC→ACC)	eutE ←	putative aldehyde dehydrogenase, ethanolamine utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,571,342	0	T	G	100.0%	117.7 / NA	33	N137T (AAC→ACC)	eutE	putative aldehyde dehydrogenase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/17);  total (16/17)

CGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGC  >  NC_000913/2571266‑2571419                                                                             |                                                                              cgGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGt                                                                           <  1:438789/81‑1 (MQ=255) cgGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGt                                                                           >  1:438833/1‑81 (MQ=255)  ggATGCGGGGCAAAAATGACGCTGTTGCCCGCGGTAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTg                                                                          <  2:438790/81‑1 (MQ=255)   gATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTg                                                                          >  1:438855/1‑80 (MQ=255)    aTGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTgg                                                                         >  2:438842/1‑80 (MQ=255)    aTGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTgg                                                                         >  1:438853/1‑80 (MQ=255)      gCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAg                                                                      >  1:438840/1‑81 (MQ=255)       cGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAg                                                                      <  1:438791/80‑1 (MQ=255)        ggggCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGc                                                                    <  2:438792/81‑1 (MQ=255)                aaTGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCg                                                             >  1:438835/1‑80 (MQ=255)                      gCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGccac                                                      >  2:438851/1‑81 (MQ=255)                          ttGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCcaccac                                                   <  1:438793/80‑1 (MQ=255)                            gCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGcc                                                >  1:438838/1‑81 (MQ=255)                             ctcgcgGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGccc                                               >  2:438852/3‑81 (MQ=255)                               cgcgGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCa                                             <  2:438794/81‑1 (MQ=255)                                    cAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTg                                        >  2:438856/1‑81 (MQ=255)                                     aaTCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGc                                       >  1:438847/1‑81 (MQ=255)                                         aGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGtt                                    <  2:438795/80‑1 (MQ=255)                                             tGATGGCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCaa                               >  2:438844/1‑81 (MQ=255)                                                  gCGTTGTTAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAg                           <  2:438796/80‑1 (MQ=255)                                                    gttgttAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGt                        >  1:438843/1‑81 (MQ=255)                                                     ttgttAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTc                       <  2:438797/81‑1 (MQ=255)                                                     ttgttAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTc                       <  2:438798/81‑1 (MQ=255)                                                        ttAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAgg                    >  2:438867/1‑81 (MQ=255)                                                        ttAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAg                     <  1:438799/80‑1 (MQ=255)                                                        ttAATTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAg                     <  2:438800/80‑1 (MQ=255)                                                          aaTTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGcc                  >  1:438864/1‑81 (MQ=255)                                                           aTTACGGTTGCCGCCGGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCg                 <  2:438801/81‑1 (MQ=255)                                                                    gccgccGGGGTAGTGGAAGGCGTCACCGAAGCCAACACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGc          >  2:438869/1‑79 (MQ=255)                                                                     ccgccgGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCa        <  2:438803/80‑1 (MQ=255)                                                                     ccgccgGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCa        <  1:438802/80‑1 (MQ=255)                                                                      cgccgGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGt      <  1:438804/81‑1 (MQ=255)                                                                        ccgGGGTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCa    <  2:438805/81‑1 (MQ=255)                                                                          ggggTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAg   >  1:438857/1‑80 (MQ=255)                                                                          ggggTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAg   >  1:438848/1‑80 (MQ=255)                                                                          ggggTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGc  <  2:438806/81‑1 (MQ=255)                                                                             |                                                                              CGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGC  >  NC_000913/2571266‑2571419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑