Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,860	A→T	C48* (TGT→TGA)	eutQ ←	putative ethanolamine utilization protein EutQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,860	0	A	T	100.0%	103.9 / NA	30	C48* (TGT→TGA)	eutQ	putative ethanolamine utilization protein EutQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/10);  total (20/10)

TCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCAC  >  NC_000913/2574783‑2574937                                                                              |                                                                              tCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCAt                                                                            >  1:439660/1‑81 (MQ=255)   gcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTc                                                                          >  2:439644/1‑81 (MQ=255)   gcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTc                                                                          >  2:439643/1‑81 (MQ=255)          cgcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGAt                                                                    <  2:439614/80‑1 (MQ=255)            cTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATgg                                                                  >  2:439658/1‑80 (MQ=255)               gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaaa                                                              <  1:439615/81‑1 (MQ=255)                   tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc                                                           <  1:439617/80‑1 (MQ=255)                   tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc                                                           <  1:439618/80‑1 (MQ=255)                   tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAc                                                            <  2:439616/79‑1 (MQ=255)                    cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc                                                           >  2:439663/1‑79 (MQ=255)                      gTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGt                                                       <  2:439619/81‑1 (MQ=255)                              ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGc                                               >  1:439655/1‑81 (MQ=255)                                  ggCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACt                                           >  2:439654/1‑81 (MQ=255)                                     aCGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCa                                        >  1:439657/1‑81 (MQ=255)                                          ggCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGc                                   >  2:439661/1‑81 (MQ=255)                                          ggCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGc                                   >  1:439664/1‑81 (MQ=255)                                            cGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCtt                                 >  1:439674/1‑81 (MQ=255)                                             gggCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTc                                <  1:439620/81‑1 (MQ=255)                                             gggCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTc                                <  2:439621/81‑1 (MQ=255)                                                cACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCgg                             <  2:439622/81‑1 (MQ=255)                                                        ccGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGtaataa                     <  2:439623/81‑1 (MQ=255)                                                            tACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCt                 >  2:439676/1‑81 (MQ=255)                                                            tACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCt                 >  1:439673/1‑81 (MQ=255)                                                            tACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCt                 >  1:439670/1‑81 (MQ=255)                                                                gggATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcg             >  1:439669/1‑81 (MQ=255)                                                                  gATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg           >  1:439666/1‑81 (MQ=255)                                                                    tCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg           >  2:439659/1‑79 (MQ=255)                                                                    tCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg           >  2:439672/1‑79 (MQ=255)                                                                     cGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg           >  2:439656/1‑78 (MQ=255)                                                                     cGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg           >  2:439675/1‑78 (MQ=255)                                                                           atctCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCAc  >  1:439665/1‑81 (MQ=255)                                                                              |                                                                              TCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCAC  >  NC_000913/2574783‑2574937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑