Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,578,163	T→C	N72S (AAC→AGC)	maeB ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,578,163	0	T	C	100.0%	79.6 / NA	23	N72S (AAC→AGC)	maeB	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

ACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGA  >  NC_000913/2578091‑2578237                                                                         |                                                                           aCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATc                                                                    <  1:440207/81‑1 (MQ=255)   gCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCAc                                                                  >  1:440254/1‑81 (MQ=255)        tGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCTCTAGAGATCACCGcca                                                             >  2:440226/1‑81 (MQ=255)           cTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATcaccaccac                                                            >  2:440245/1‑79 (MQ=255)           cTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGccacca                                                          <  2:440208/81‑1 (MQ=255)               cATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGtt                                                      <  1:440209/81‑1 (MQ=255)                          tgcctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCg                                            <  2:440210/80‑1 (MQ=255)                            cctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGt                                          <  1:440211/80‑1 (MQ=255)                             ctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat                                        <  1:440212/81‑1 (MQ=255)                             ctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat                                        <  1:440213/81‑1 (MQ=255)                               gccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat                                      >  2:440238/1‑81 (MQ=255)                                       gcCAATGTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGc                              >  1:440244/1‑81 (MQ=255)                                             gTTGCCTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTaa                        >  1:440241/1‑81 (MQ=255)                                                 ccTAACCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACgg                     <  1:440214/80‑1 (MQ=255)                                                     aCCCCAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtt                >  1:440239/1‑81 (MQ=255)                                                       cccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtttt              >  1:440243/1‑81 (MQ=255)                                                        ccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt             <  1:440217/81‑1 (MQ=255)                                                        ccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt             <  1:440216/81‑1 (MQ=255)                                                        ccAGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt             <  1:440215/81‑1 (MQ=255)                                                          aGCACCGCCGTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCg           <  2:440218/81‑1 (MQ=255)                                                              ccgccgTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt       >  1:440249/1‑81 (MQ=255)                                                                gccgTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATGTc      >  2:440251/1‑80 (MQ=255)                                                                   gTACCGCTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGa  >  1:440235/1‑81 (MQ=255)                                                                         |                                                                           ACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGA  >  NC_000913/2578091‑2578237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑