Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,582,923	T→C	T186A (ACG→GCG)	nudK ←	GDP‑mannose hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,582,923	0	T	C	100.0%	66.8 / NA	20	T186A (ACG→GCG)	nudK	GDP‑mannose hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

AATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCT  >  NC_000913/2582848‑2582998                                                                            |                                                                            aaTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGc                                                                         >  1:441146/1‑80 (MQ=255)  aTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCaa                                                                       <  1:441125/81‑1 (MQ=255)     ggATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATa                                                                    <  2:441126/81‑1 (MQ=255)           gTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTaa                                                               <  1:441127/80‑1 (MQ=255)            tCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAg                                                              >  2:441152/1‑80 (MQ=255)                        aataatAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTc                                                 <  2:441128/81‑1 (MQ=255)                            ataaACAACGAAATATTATTGTTATTTTCAAGTCCATTAAAGGTGACGCTTGCAAATAGTTAAGCAATAAACCCGCCTTAc                                             >  1:441154/1‑81 (MQ=255)                               aacaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGt                                          <  2:441129/81‑1 (MQ=255)                                   acGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACg                                      <  2:441130/81‑1 (MQ=255)                                    cGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACg                                      >  2:441162/1‑80 (MQ=255)                                    cGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGt                                     <  2:441131/81‑1 (MQ=255)                                      aaaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTAt                                   <  1:441132/81‑1 (MQ=255)                                            tattGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGcc                             >  2:441171/1‑81 (MQ=255)                                             attGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGcc                             <  1:441133/80‑1 (MQ=255)                                                 ttATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGttt                        >  2:441153/1‑81 (MQ=255)                                                     ttttCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGat                    >  2:441150/1‑81 (MQ=255)                                                        tCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGatcat                 >  1:441155/1‑81 (MQ=255)                                                             ccATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCAtctc              >  2:441170/1‑79 (MQ=255)                                                                   aaTGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCt      <  1:441134/81‑1 (MQ=255)                                                                        gACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCt  >  1:441156/1‑80 (MQ=255)                                                                            |                                                                            AATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCT  >  NC_000913/2582848‑2582998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑