Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,604,683	A→G	S279S (TCA→TCG)	hyfC →	hydrogenase 4 component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,604,683	0	A	G	100.0%	147.0 / NA	41	S279S (TCA→TCG)	hyfC	hydrogenase 4 component C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/23);  total (18/23)

TTCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGC  >  NC_000913/2604611‑2604760                                                                         |                                                                              ttCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAg                                                                        >  2:445299/1‑80 (MQ=255)   ctctcGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGaa                                                                      <  2:445241/80‑1 (MQ=255)      tcGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAaca                                                                  >  1:445285/1‑81 (MQ=255)       cGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAacac                                                                 >  2:445293/1‑81 (MQ=255)          ctgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCt                                                              <  2:445243/81‑1 (MQ=255)          ctgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCt                                                              <  2:445242/81‑1 (MQ=255)           tgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTg                                                             >  1:445287/1‑81 (MQ=255)           tgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTg                                                             >  1:445305/1‑81 (MQ=255)             cctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTgg                                                            <  2:445244/80‑1 (MQ=255)             cctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGc                                                           <  2:445245/81‑1 (MQ=255)                 ctgACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCAcgc                                                       <  2:445247/81‑1 (MQ=255)                 ctgACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCAc                                                         <  2:445246/79‑1 (MQ=255)                        cACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGtt                                                >  2:445307/1‑81 (MQ=255)                         aCTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGttt                                               >  2:445290/1‑81 (MQ=255)                          cTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGtttt                                              <  1:445248/81‑1 (MQ=255)                             gTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTa                                           >  1:445302/1‑81 (MQ=255)                                gTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACt                                         >  1:445295/1‑80 (MQ=255)                                gTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACt                                         >  1:445315/1‑80 (MQ=255)                                gTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTc                                        <  1:445249/81‑1 (MQ=255)                                   aCGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCAtt                                     >  1:445296/1‑81 (MQ=255)                                   aCGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCAtt                                     >  2:445313/1‑81 (MQ=255)                                   aCGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCAt                                      <  1:445250/80‑1 (MQ=255)                                    cGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTc                                    <  1:445251/81‑1 (MQ=255)                                        gctCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCa                                >  1:445292/1‑81 (MQ=255)                                          tCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCAtg                              >  1:445310/1‑81 (MQ=255)                                              ggTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGAc                          <  1:445252/81‑1 (MQ=255)                                              ggTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGAc                          <  1:445253/81‑1 (MQ=255)                                                  ttGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtgg                      >  1:445298/1‑81 (MQ=255)                                                  ttGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtgg                      >  1:445286/1‑81 (MQ=255)                                                  ttGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtgg                      >  2:445294/1‑81 (MQ=255)                                                    gCTGATTTTTGTACTGGCCTCGATCGCAGAGAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtggc                     <  2:445254/80‑1 (MQ=255)                                                     cTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtggct                    <  2:445255/80‑1 (MQ=255)                                                            ttGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTc             <  1:445256/80‑1 (MQ=255)                                                             tGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCa            <  2:445257/80‑1 (MQ=255)                                                              gTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGc          >  1:445303/1‑81 (MQ=255)                                                               tACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGcc         <  2:445258/81‑1 (MQ=255)                                                               tACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGc          <  2:445259/80‑1 (MQ=255)                                                                 cTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCtt       <  2:445260/81‑1 (MQ=255)                                                                 cTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCt        <  2:445261/80‑1 (MQ=255)                                                                     ccTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTtgctg   <  2:445262/81‑1 (MQ=255)                                                                      cTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTtgctgc  <  2:445263/81‑1 (MQ=255)                                                                         |                                                                              TTCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGC  >  NC_000913/2604611‑2604760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑