Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,485	A→G	I56I (ATT→ATC)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,485	0	A	G	100.0%	100.8 / NA	29	I56I (ATT→ATC)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/16);  total (13/16)

TTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATC  >  NC_000913/2638409‑2638560                                                                             |                                                                            ttACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGAt                                                                           <  2:451615/79‑1 (MQ=255) ttACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGAt                                                                           <  2:451616/79‑1 (MQ=255)  tACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGcc                                                                        >  1:451641/1‑81 (MQ=255)  tACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGcc                                                                        >  1:451658/1‑81 (MQ=255)   aCTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGcc                                                                        <  2:451617/80‑1 (MQ=255)   aCTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCg                                                                       >  1:451652/1‑81 (MQ=255)           aGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAAc                                                               <  2:451618/81‑1 (MQ=255)                    cGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCt                                                      >  1:451667/1‑81 (MQ=255)                     gCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTc                                                     <  1:451619/81‑1 (MQ=255)                        gCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCTGATGCCGCTACCAACGGAAGTGCTCCAc                                                  >  2:451668/1‑81 (MQ=255)                             gACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGt                                             <  2:451620/81‑1 (MQ=255)                              aCAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTg                                            >  2:451657/1‑81 (MQ=255)                                    ttGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCgga                                      <  2:451621/81‑2 (MQ=255)                                    ttGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGc                                      <  1:451623/81‑1 (MQ=255)                                    ttGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGc                                      <  2:451622/81‑1 (MQ=255)                                       tCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTa                                   <  2:451624/81‑1 (MQ=255)                                        ccGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTa                                   >  2:451660/1‑80 (MQ=255)                                         cGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTaaa                                 <  2:451625/81‑1 (MQ=255)                                             aGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTgg                             >  2:451673/1‑81 (MQ=255)                                               tGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGtt                           <  2:451626/81‑1 (MQ=255)                                               tGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGtt                           <  2:451627/81‑1 (MQ=255)                                                 ccGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGtttt                         <  1:451628/81‑1 (MQ=255)                                                    gATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCaa                      >  2:451659/1‑81 (MQ=255)                                                     aTGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAAc                     <  1:451629/81‑1 (MQ=255)                                                      tGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACg                    <  1:451630/81‑1 (MQ=255)                                                             tGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGc              >  2:451661/1‑80 (MQ=255)                                                              ggAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCaa            >  1:451663/1‑81 (MQ=255)                                                                    gAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGa      >  1:451662/1‑81 (MQ=255)                                                                        ttGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATc  >  1:451669/1‑81 (MQ=255)                                                                             |                                                                            TTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATC  >  NC_000913/2638409‑2638560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑