Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,677,674	C→A	D230Y (GAT→TAT)	yphE ←	putative ABC transporter ATP‑binding protein YphE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,677,674	0	C	A	100.0%	105.7 / NA	31	D230Y (GAT→TAT)	yphE	putative ABC transporter ATP‑binding protein YphE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/16);  total (15/16)

CCGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC  >  NC_000913/2677596‑2677745                                                                               |                                                                        ccGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagc                                                                       <  1:459474/81‑1 (MQ=255)   ggCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagccc                                                                     >  1:459516/1‑81 (MQ=255)    gcgcAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagcccg                                                                    >  2:459536/1‑81 (MQ=255)       cAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCa                                                                 >  2:459475/1‑81 (MQ=255)         atatCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCAcc                                                               <  1:459476/81‑1 (MQ=255)             cAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGAc                                                           >  1:459524/1‑81 (MQ=255)                cGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCAt                                                        <  2:459477/81‑1 (MQ=255)                   gATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATcgc                                                     >  2:459523/1‑81 (MQ=255)                    aTCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATcgc                                                     <  1:459478/80‑1 (MQ=255)                     tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCgcgc                                                   <  2:459479/81‑1 (MQ=255)                     tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCgcgc                                                   <  1:459480/81‑1 (MQ=255)                     tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTCCGAGCATCACATAGCCCGCCACCTGACCATCgcgc                                                   <  1:459481/81‑1 (MQ=255)                       gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCa                                                  >  2:459515/1‑80 (MQ=255)                       gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt                                                 <  1:459482/81‑1 (MQ=255)                        cgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt                                                 >  2:459522/1‑80 (MQ=255)                              aGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTg                                          >  2:459521/1‑81 (MQ=255)                               gCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTgg                                         <  2:459483/81‑1 (MQ=255)                                  tCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGcac                                      <  2:459484/81‑1 (MQ=255)                                     gCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACag                                    >  1:459528/1‑80 (MQ=255)                                     gCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACag                                    >  1:459517/1‑80 (MQ=255)                                            aaTATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAAt                            <  2:459485/81‑1 (MQ=255)                                                tgatgCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGACACAGGAGGCAATgcg                         <  2:459486/80‑1 (MQ=255)                                                 gatgCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATgcggc                       >  1:459541/1‑81 (MQ=255)                                                         gAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttct               >  1:459557/1‑81 (MQ=255)                                                         gAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttc                <  2:459487/80‑1 (MQ=255)                                                          aGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCCTAACGGTGGCACAGGAGGCAATGCGGCGAATttctt              <  1:459488/81‑1 (MQ=255)                                                           ggTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttcttc             >  2:459532/1‑81 (MQ=255)                                                            gtgtTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTcc            <  1:459489/81‑1 (MQ=255)                                                              gtTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCAt          >  1:459533/1‑81 (MQ=255)                                                                tttCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATcc        >  1:459530/1‑81 (MQ=255)                                                                 ttCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCg       >  2:459535/1‑81 (MQ=255)                                                                    gAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTg    <  2:459490/81‑1 (MQ=255)                                                                     aGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTgg   <  2:459491/81‑1 (MQ=255)                                                                      gCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGc  >  1:459525/1‑81 (MQ=255)                                                                               |                                                                        CCGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC  >  NC_000913/2677596‑2677745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑