Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,434	T→C	S202P (TCT→CCT)	nadK →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,434	0	T	C	100.0%	101.8 / NA	29	S202P (TCT→CCT)	nadK	NAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/11);  total (18/11)

ATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGC  >  NC_000913/2751360‑2751511                                                                           |                                                                              aTGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCccct                                                                             <  2:473511/77‑1 (MQ=255) aTGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTg                                                                          >  2:473565/1‑80 (MQ=255)          ttattTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTa                                                                <  1:473512/81‑1 (MQ=255)             tttCGACGCCAACAGGCTCCACCGCCTATTACCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTAccc                                                             <  1:473513/81‑1 (MQ=255)               tCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTg                                                           >  1:473559/1‑81 (MQ=255)                cGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTgg                                                          >  2:473562/1‑81 (MQ=255)                 gACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGt                                                         >  1:473552/1‑81 (MQ=255)                  aCGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTg                                                        <  2:473514/81‑1 (MQ=255)                    gCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGcc                                                      >  2:473563/1‑81 (MQ=255)                          aGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGtt                                                >  1:473570/1‑81 (MQ=255)                           ggCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTc                                               <  1:473515/81‑1 (MQ=255)                           ggCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTc                                               <  1:473516/81‑1 (MQ=255)                            gCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTcc                                              >  2:473579/1‑81 (MQ=255)                                 aCCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCa                                          >  2:473576/1‑80 (MQ=255)                                 aCCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCa                                          >  2:473560/1‑80 (MQ=255)                                      cTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGtt                                    <  2:473517/81‑1 (MQ=255)                                           cccTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCa                                <  2:473518/80‑1 (MQ=255)                                            ccTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAg                               <  2:473519/80‑1 (MQ=255)                                                tctGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGa                          >  2:473554/1‑81 (MQ=255)                                                  tGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGAc                         >  1:473564/1‑80 (MQ=255)                                                   gCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCa                       >  2:473558/1‑81 (MQ=255)                                                    cAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGAc                         <  2:473520/78‑1 (MQ=255)                                                     aGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACt                     >  1:473569/1‑81 (MQ=255)                                                     aGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTCTCAGCACGACCACt                     >  2:473557/1‑81 (MQ=255)                                                      ggCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTg                    >  1:473561/1‑81 (MQ=255)                                                             cTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATa              >  1:473574/1‑80 (MQ=255)                                                                   tGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcag         >  2:473583/1‑79 (MQ=255)                                                                    gACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagcag      >  2:473580/1‑81 (MQ=255)                                                                     aCCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagcag      <  1:473522/80‑1 (MQ=255)                                                                     aCCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagc        <  1:473521/78‑1 (MQ=255)                                                                       ccccccTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagcagcag   <  2:473523/81‑1 (MQ=255)                                                                       ccccccTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagcagcag   >  1:473575/1‑81 (MQ=255)                                                                        ccccCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACagcagcagc  <  2:473524/81‑1 (MQ=255)                                                                           |                                                                              ATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGC  >  NC_000913/2751360‑2751511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑