Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,758,263	T→C	E113G (GAA→GGA)	yfjH ←	CP4‑57 prophage; protein YfjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,758,263	0	T	C	100.0%	78.5 / NA	23	E113G (GAA→GGA)	yfjH	CP4‑57 prophage; protein YfjH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

AAAACGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCT  >  NC_000913/2758186‑2758333                                                                              |                                                                       aaaaCGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCt                                                                     >  1:474645/1‑81 (MQ=255)               gTAAGGGTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGc                                                       <  1:474612/81‑1 (MQ=255)                    gTTTGAACTTAGGTTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCAtt                                                  <  2:474613/81‑1 (MQ=255)                       tGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCa                                                <  2:474614/80‑1 (MQ=255)                         aaCTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTg                                             >  1:474638/1‑81 (MQ=255)                            ttAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTAt                                          >  2:474650/1‑81 (MQ=255)                               gATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGt                                       >  1:474642/1‑81 (MQ=255)                                  ttttCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGcc                                    >  2:474633/1‑81 (MQ=255)                                    ttCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCtt                                  >  2:474656/1‑81 (MQ=255)                                     tCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTg                                 >  2:477095/1‑81 (MQ=255)                                      ccAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGt                                >  2:474647/1‑81 (MQ=255)                                        aGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGttt                              <  2:474615/81‑1 (MQ=255)                                         gCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTc                             >  1:474641/1‑81 (MQ=255)                                           aTTCTTGAGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTcc                            >  1:474640/1‑80 (MQ=255)                                                  aGCTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTa                     <  1:474616/80‑1 (MQ=255)                                                    cTGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACAt                  <  1:474617/81‑1 (MQ=255)                                                     tGGATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATc                 >  2:474648/1‑81 (MQ=255)                                                      ggATCATATAATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCg                >  1:474643/1‑81 (MQ=255)                                                            tataATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGaa          >  2:474654/1‑81 (MQ=255)                                                              taATTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAat         <  1:474618/80‑1 (MQ=255)                                                                aTTCAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAAtata      <  2:474619/81‑1 (MQ=255)                                                                   cAGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAAc    <  2:474620/80‑1 (MQ=255)                                                                    aGTGTATTCTCCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCt  <  1:474621/81‑1 (MQ=255)                                                                              |                                                                       AAAACGTTTCCTTGGTAAGGTTTGAACTTAGATTTTTCCAGCATTCTTGAGCTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCT  >  NC_000913/2758186‑2758333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑