Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,767,815	G→A	E36K (GAG→AAG)	yfjP →	CP4‑57 prophage; putative GTP‑binding protein YfjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,767,815	0	G	A	100.0%	66.8 / NA	20	E36K (GAG→AAG)	yfjP	CP4‑57 prophage; putative GTP‑binding protein YfjP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/6);  total (14/6)

ACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTT  >  NC_000913/2767739‑2767880                                                                             |                                                                  aCAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGc                                                                >  2:476055/1‑80 (MQ=255)         cccTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCg                                                       >  2:476042/1‑81 (MQ=255)              tCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGcat                                                   <  1:476019/80‑1 (MQ=255)                cGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGcatcat                                                >  2:476052/1‑81 (MQ=255)                cGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGcatcat                                                <  2:476020/81‑1 (MQ=255)                  gTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATgg                                              >  1:476050/1‑81 (MQ=255)                      aCCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTa                                           >  2:476040/1‑80 (MQ=255)                               ggTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGc                                 <  1:476021/81‑1 (MQ=255)                                  aTCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCgg                               >  1:476054/1‑80 (MQ=255)                                       aaCGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAaga                         <  1:476022/81‑1 (MQ=255)                                         cGTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAagag                        >  2:476057/1‑80 (MQ=255)                                          gTATATTGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAagag                        >  2:476074/1‑79 (MQ=255)                                               ttGCAGCAGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCtt                 >  2:476058/1‑81 (MQ=255)                                                 gcagcaGATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTg               >  1:476049/1‑81 (MQ=255)                                                  cagcagATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTg               >  1:476051/1‑80 (MQ=255)                                                   agcagATAAATCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCa             <  2:476023/81‑1 (MQ=255)                                                          aaaTCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCt      >  1:476064/1‑81 (MQ=255)                                                            aTCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGt    <  1:476024/81‑1 (MQ=255)                                                             tCAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGtt   >  2:476067/1‑81 (MQ=255)                                                              cAGTTAACCCACTACAAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGttt  >  2:476059/1‑81 (MQ=255)                                                                             |                                                                  ACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTT  >  NC_000913/2767739‑2767880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑