Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,782,394	G→A	D111D (GAC→GAT)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,782,394	0	G	A	100.0%	46.9 / NA	15	D111D (GAC→GAT)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/9);  total (6/9)

GTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGA  >  NC_000913/2782320‑2782460                                                                           |                                                                   gTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGaa                                                             <  1:478431/81‑1 (MQ=255)    ccATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAAtt                                                           >  1:478462/1‑80 (MQ=255)       ttGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACt                                                       >  1:478458/1‑81 (MQ=255)             aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGc                                                 >  1:478457/1‑81 (MQ=255)                 ccccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCAcc                                             <  1:478432/81‑1 (MQ=255)                          aaaCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGt                                    >  2:478460/1‑81 (MQ=255)                             cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGt                                 <  1:478433/81‑1 (MQ=255)                                 cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                             <  1:478434/81‑1 (MQ=255)                                    aTTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGtattat                            >  1:478461/1‑79 (MQ=255)                                       gCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                        <  1:478435/80‑1 (MQ=255)                                       gCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                        <  1:478436/80‑1 (MQ=255)                                       gCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                        <  2:478437/80‑1 (MQ=255)                                                 aTTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaa             <  1:478438/81‑1 (MQ=255)                                                          ccTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa     <  1:478439/80‑1 (MQ=255)                                                            tgtgGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa  >  2:478464/1‑81 (MQ=255)                                                                           |                                                                   GTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGA  >  NC_000913/2782320‑2782460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑