Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,836,333	G→A	R365C (CGC→TGC)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,836,333	0	G	A	100.0%	125.0 / NA	36	R365C (CGC→TGC)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/14);  total (22/14)

TTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAT  >  NC_000913/2836258‑2836407                                                                            |                                                                           ttttAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCAcacca                                                                       >  1:488368/1‑81 (MQ=255)   ttAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACaccacc                                                                     >  2:488373/1‑81 (MQ=255)        gCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatc                                                                <  2:488329/81‑1 (MQ=255)        gCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAat                                                                 >  2:488363/1‑80 (MQ=255)        gCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAat                                                                 >  1:488391/1‑80 (MQ=255)         cccGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatca                                                               >  1:488382/1‑81 (MQ=255)           cGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatcatc                                                             <  2:488330/81‑1 (MQ=255)            ggAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAatcatc                                                             >  2:488376/1‑80 (MQ=255)              aGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATcatccat                                                          >  1:488372/1‑81 (MQ=255)                  aaaGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGc                                                      >  1:488379/1‑81 (MQ=255)                  aaaGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGc                                                      >  1:488390/1‑81 (MQ=255)                      ccAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCa                                                  >  2:488371/1‑81 (MQ=255)                       cAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCAc                                                 <  1:488331/81‑1 (MQ=255)                       cAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCAc                                                 >  2:488358/1‑81 (MQ=255)                        aGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCAc                                                 >  1:488384/1‑80 (MQ=255)                          cgcATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGAt                                              <  1:488332/81‑1 (MQ=255)                           gcATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATg                                             >  2:488386/1‑81 (MQ=255)                             aTCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTc                                           <  1:488333/81‑1 (MQ=255)                             aTCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTc                                           >  1:488369/1‑81 (MQ=255)                                cGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTcgc                                         <  1:488334/80‑1 (MQ=255)                                 ggCACATACCCCCGCGAACGGCGCAGCATTCCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCgg                                       >  1:488380/1‑81 (MQ=255)                                  gCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGt                                      <  1:488335/81‑1 (MQ=255)                                   cacaTACCCCCGCGAACGGCGCAGCATGTCGCCGCGTTCGCACAACACCGAATCATCCATCCGCTGCACGATGTCGCGGtt                                     <  2:488336/81‑1 (MQ=255)                                       tACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATg                                  >  2:488374/1‑80 (MQ=255)                                            ccGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATc                              >  2:488383/1‑79 (MQ=255)                                            ccGCGAACGGCGCAGCATTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCaa                            <  1:488337/81‑1 (MQ=255)                                                       gcagcaTTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCgg                 <  2:488338/81‑1 (MQ=255)                                                       gcagcaTTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCgg                 >  2:488375/1‑81 (MQ=255)                                                         agcaTTTCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCa               <  2:488339/81‑1 (MQ=255)                                                               tCGCCGCTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGccc         >  2:488394/1‑81 (MQ=255)                                                                cgccgcTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGccc         <  2:488340/80‑1 (MQ=255)                                                                 gccgcTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCt        <  2:488341/80‑1 (MQ=255)                                                                  ccgcTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTg       >  2:488399/1‑80 (MQ=255)                                                                     cTTTCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCaaa   >  1:488401/1‑81 (MQ=255)                                                                      tttCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAt  >  1:488370/1‑81 (MQ=255)                                                                        tCGCACACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAt  <  1:488342/79‑1 (MQ=255)                                                                            |                                                                           TTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAAT  >  NC_000913/2836258‑2836407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑