Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,802	G→A	E4K (GAA→AAA)	yahH →	putative uncharacterized protein YahH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,802	0	G	A	100.0%	80.6 / NA	25	E4K (GAA→AAA)	yahH	putative uncharacterized protein YahH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

GCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGG  >  NC_000913/339724‑339878                                                                               |                                                                             gCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAaaaa                                                                            >  1:66013/1‑81 (MQ=255)  cGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAaaaa                                                                            <  1:65980/80‑1 (MQ=255)   gAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATc                                                                          >  2:66020/1‑81 (MQ=255)    aaCGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCt                                                                         >  2:66032/1‑81 (MQ=255)    aaCGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCt                                                                         <  2:65981/81‑1 (MQ=255)       gTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTg                                                                        >  1:66028/1‑79 (MQ=255)              cGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGa                                                               >  2:66033/1‑81 (MQ=255)              cGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGa                                                               >  1:66019/1‑81 (MQ=255)               gTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGa                                                               >  1:66026/1‑80 (MQ=255)                    aCCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAg                                                           <  2:65982/79‑1 (MQ=255)                    aCCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGc                                                          <  1:65983/80‑1 (MQ=255)                        aCATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAgc                                                     >  1:66027/1‑81 (MQ=255)                          aTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAgcg                                                    >  1:66024/1‑80 (MQ=255)                               cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCa                                               <  1:65984/80‑1 (MQ=17)                               cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAg                                              >  1:66040/1‑81 (MQ=17)                                                gAACGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGc                             >  2:66036/1‑81 (MQ=255)                                                 aaCGCCTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGc                             <  1:65986/80‑1 (MQ=255)                                                      cTTATCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATg                       >  2:66042/1‑81 (MQ=255)                                                          tCCGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATgcggc                   <  2:65987/81‑1 (MQ=255)                                                            cGGCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGt                 <  2:65988/81‑1 (MQ=255)                                                              gCCTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTg                >  2:66039/1‑80 (MQ=255)                                                                cTACGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAAc             <  2:65989/81‑1 (MQ=255)                                                                   cGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGcc          >  1:66031/1‑81 (MQ=255)                                                                   cGAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGcc          <  2:65990/81‑1 (MQ=255)                                                                    gAATGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCt         >  2:66041/1‑81 (MQ=255)                                                                     aaTGGCGCAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCtt        <  1:65991/81‑1 (MQ=37)                                                                           gcAAAAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCgg  >  2:66054/1‑81 (MQ=17)                                                                               |                                                                             GCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGG  >  NC_000913/339724‑339878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑