Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,894,763	T→A	L190I (TTA→ATA)	ygcP →	putative anti‑terminator regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,894,763	0	T	A	100.0%	123.5 / NA	35	L190I (TTA→ATA)	ygcP	putative anti‑terminator regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/18);  total (17/18)

GATGCGCGTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCG  >  NC_000913/2894685‑2894838                                                                               |                                                                            gaTGCGCGTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATa                                                                           >  1:499825/1‑81 (MQ=255)          aaTGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACgg                                                                  >  2:499819/1‑81 (MQ=255)          aaTGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACgg                                                                  >  1:499820/1‑81 (MQ=255)              cGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGAt                                                               >  1:499826/1‑80 (MQ=255)              cGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATa                                                              <  2:499776/81‑1 (MQ=255)                aTTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAAt                                                            <  1:499777/81‑1 (MQ=255)                   aaCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCg                                                          >  1:499804/1‑80 (MQ=255)                       cgGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAAc                                                      >  2:499823/1‑80 (MQ=255)                           tgtCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAAtt                                                 <  2:499778/81‑1 (MQ=255)                             tCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGa                                               <  2:499779/81‑1 (MQ=255)                                tGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAAtt                                            >  1:499824/1‑81 (MQ=255)                                  gcgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTg                                          <  2:499780/81‑1 (MQ=255)                                    gcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGt                                        <  1:499781/81‑1 (MQ=255)                                     cTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGtt                                       <  2:499782/81‑1 (MQ=255)                                         ccaccaCGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTgct                                   >  2:499836/1‑79 (MQ=255)                                           accacGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGAttt                                 <  1:499783/81‑1 (MQ=255)                                               cGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAg                             <  1:499784/81‑1 (MQ=255)                                                gAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAgg                            >  1:499834/1‑81 (MQ=255)                                                  aTACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGcc                          <  1:499785/81‑1 (MQ=255)                                                    aCCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCg                         >  2:499831/1‑80 (MQ=255)                                                            cTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCcaca                >  1:499835/1‑81 (MQ=255)                                                            cTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCcaca                >  1:499827/1‑81 (MQ=255)                                                              ggACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATc              >  1:499832/1‑81 (MQ=255)                                                               gACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACAt               <  1:499788/79‑1 (MQ=255)                                                               gACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATcc             <  1:499787/81‑1 (MQ=255)                                                               gACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATcc             <  2:499790/81‑1 (MQ=255)                                                               gACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATc              <  2:499789/80‑1 (MQ=255)                                                               gACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATc              <  2:499786/80‑1 (MQ=255)                                                                aCGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATcc             >  1:499847/1‑80 (MQ=255)                                                                aCGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATcc             >  1:499844/1‑80 (MQ=255)                                                                aCGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCg            <  2:499791/81‑1 (MQ=255)                                                                   ttAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACa         <  2:499792/81‑1 (MQ=255)                                                                     aGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACAtt       >  1:499828/1‑81 (MQ=255)                                                                      gCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACAtt       >  1:499842/1‑80 (MQ=255)                                                                         aaaaaaATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAgc   <  1:499793/81‑1 (MQ=255)                                                                          aaaaaaTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAgcg  <  2:499794/81‑1 (MQ=255)                                                                               |                                                                            GATGCGCGTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCG  >  NC_000913/2894685‑2894838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑