Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,373	C→T	V16I (GTT→ATT)	ygcU ←	putative FAD‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,373	0	C	T	100.0%	70.0 / NA	21	V16I (GTT→ATT)	ygcU	putative FAD‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/12);  total (9/12)

AATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGATAATAAAAATGGA  >  NC_000913/2899303‑2899447                                                                       |                                                                           aaTTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCttt                                                                  <  1:500765/81‑1 (MQ=255)     tACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCt                                                              >  2:500793/1‑81 (MQ=255)       cGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTgg                                                            <  1:500766/81‑1 (MQ=255)            aCGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGa                                                        >  1:500785/1‑80 (MQ=255)             cGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACa                                                      <  2:500767/81‑1 (MQ=255)              ggTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACaa                                                     >  1:500787/1‑81 (MQ=255)              ggTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACaa                                                     >  1:500797/1‑81 (MQ=255)                tCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATc                                                   <  1:500768/81‑1 (MQ=255)                           tttCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCgag                                         <  2:500769/80‑1 (MQ=255)                             tCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGAt                                       <  2:500770/80‑1 (MQ=255)                                 tAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAg                                   <  1:500771/80‑1 (MQ=255)                                    tACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACAt                               <  1:500772/81‑1 (MQ=255)                                         tttCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTaa                          >  2:500794/1‑81 (MQ=255)                                            cATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttc                       >  2:500804/1‑81 (MQ=255)                                               cGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttcctt                    >  2:500796/1‑81 (MQ=255)                                                ggTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttccttc                   >  2:500792/1‑81 (MQ=255)                                                ggTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttcctt                    <  1:500773/80‑1 (MQ=255)                                                     ttACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTTat              <  2:500774/81‑1 (MQ=255)                                                         gcgATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGataata          <  1:500775/81‑1 (MQ=255)                                                         gcgATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGataat           >  1:500800/1‑80 (MQ=255)                                                                 cACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGATAATAAAAATGGa  <  2:500776/81‑1 (MQ=255)                                                                       |                                                                           AATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGATAATAAAAATGGA  >  NC_000913/2899303‑2899447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑