Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,489	C→T	M151I (ATG→ATA)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,489	0	C	T	100.0%	95.8 / NA	28	M151I (ATG→ATA)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/8);  total (20/8)

TTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCG  >  NC_000913/2907411‑2907556                                                                               |                                                                    tttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTttat                                                                   >  2:501905/1‑81 (MQ=255)    cgcgCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTttatca                                                                 <  1:501863/80‑1 (MQ=255)              ggCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGc                                                      <  1:501864/81‑1 (MQ=255)               gCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa                                                     >  2:501900/1‑81 (MQ=255)               gCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa                                                     >  1:501899/1‑81 (MQ=255)                cTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCAt                                                    <  1:501865/81‑1 (MQ=255)                 tGAGTCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa                                                     >  2:501891/1‑79 (MQ=255)                  gAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa                                                     >  1:501903/1‑78 (MQ=255)                   aaTCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATag                                                  <  1:501866/80‑1 (MQ=255)                     tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATagag                                                >  1:501906/1‑80 (MQ=255)                         gAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTAttt                                           >  2:501918/1‑81 (MQ=255)                           aTTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGc                                         <  1:501867/81‑1 (MQ=255)                                  gATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGt                                  <  1:501868/81‑1 (MQ=255)                                       cAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgt                             >  1:501907/1‑81 (MQ=255)                                         aCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttc                           <  2:501869/81‑1 (MQ=255)                                         aCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgtt                            >  1:501911/1‑80 (MQ=255)                                            ttgttgTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagt                        >  1:501924/1‑81 (MQ=255)                                             tgttgtCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagtt                       >  1:501922/1‑81 (MQ=255)                                                  tCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGc                   >  2:501910/1‑80 (MQ=255)                                                        tGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGc            >  2:501914/1‑81 (MQ=255)                                                         gCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCt           >  2:501909/1‑81 (MQ=255)                                                            caccacCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCg         >  2:501923/1‑80 (MQ=255)                                                             accaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCg         <  2:501870/79‑1 (MQ=255)                                                             accaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa       >  1:501920/1‑81 (MQ=255)                                                              ccaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa       >  1:501919/1‑80 (MQ=255)                                                              ccaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTAc      <  2:501871/81‑1 (MQ=255)                                                               caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa       >  2:501913/1‑79 (MQ=255)                                                               caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa       >  2:501965/1‑79 (MQ=255)                                                                accGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAg    >  1:501901/1‑81 (MQ=255)                                                                  cGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCg  >  1:501925/1‑81 (MQ=255)                                                                               |                                                                    TTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCG  >  NC_000913/2907411‑2907556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑