Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,958,495	A→G	Y130Y (TAT→TAC)	ptrA ←	protease 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,958,495	0	A	G	100.0%	87.4 / NA	25	Y130Y (TAT→TAC)	ptrA	protease 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/10);  total (15/10)

TGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGATAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGC  >  NC_000913/2958419‑2958563                                                                             |                                                                     tGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAg                                                                   >  1:511984/1‑80 (MQ=255) tGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAg                                                                   >  1:512022/1‑80 (MQ=255)   tCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGa                                                                  >  2:512038/1‑79 (MQ=255)    cGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAg                                                               >  2:512035/1‑81 (MQ=255)     gAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGc                                                              >  2:667719/1‑81 (MQ=255)         aaaGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTg                                                          >  1:512014/1‑81 (MQ=255)         aaaGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTg                                                          >  1:512024/1‑81 (MQ=255)         aaaGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTg                                                          <  2:511985/81‑1 (MQ=255)                       aGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGt                                            >  2:512040/1‑81 (MQ=255)                          aTCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCt                                         >  1:512045/1‑81 (MQ=255)                              gCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCa                                     >  2:512027/1‑81 (MQ=255)                                cAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCAtt                                   <  2:511987/81‑1 (MQ=255)                                cAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCAtt                                   <  2:511986/81‑1 (MQ=255)                                 aGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATtg                                  <  1:511988/81‑1 (MQ=255)                                   gCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATtgtg                                <  2:511989/81‑1 (MQ=255)                                      gTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGAc                              >  1:512025/1‑80 (MQ=255)                                            cGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAccgcc                       <  1:511990/81‑1 (MQ=255)                                                      aaGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtttt              >  1:512041/1‑80 (MQ=255)                                                        ggCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTgag           <  2:511991/81‑1 (MQ=255)                                                              gtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATAtt      <  1:511992/80‑1 (MQ=255)                                                              gtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTc     >  1:512048/1‑81 (MQ=255)                                                              gtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTc     <  2:511993/81‑1 (MQ=255)                                                              gtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTc     >  2:512034/1‑81 (MQ=255)                                                                tctcAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCgg   >  1:512032/1‑81 (MQ=255)                                                                    aaCTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGc  <  1:511994/78‑1 (MQ=255)                                                                             |                                                                     TGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGATAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGC  >  NC_000913/2958419‑2958563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑