Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,975,617	A→G	I133T (ATT→ACT)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,975,617	0	A	G	100.0%	126.7 / NA	36	I133T (ATT→ACT)	aas	acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/21);  total (15/21)

CGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAT  >  NC_000913/2975541‑2975693                                                                             |                                                                             cGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAAc                                                                          <  2:515495/81‑1 (MQ=255) cGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAAc                                                                          >  2:515550/1‑81 (MQ=255)         aGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCAc                                                                  >  2:515556/1‑81 (MQ=255)            gACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCAc                                                                  >  2:515546/1‑78 (MQ=255)                 ttAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTc                                                          >  2:515528/1‑81 (MQ=255)                  tAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTc                                                          <  1:515496/80‑1 (MQ=255)                    aCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgcc                                                       >  2:515560/1‑81 (MQ=255)                    aCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgc                                                        <  1:515497/80‑1 (MQ=255)                    aCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgc                                                        <  1:515498/80‑1 (MQ=255)                      cAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTcgccgc                                                     <  2:515499/81‑1 (MQ=255)                        gACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGa                                                   >  1:515557/1‑81 (MQ=255)                         aCCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGAc                                                  <  1:515500/81‑1 (MQ=255)                          ccTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACa                                                 <  1:515501/81‑1 (MQ=255)                              tCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACaaa                                               <  1:515502/79‑1 (MQ=255)                               cAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAAcc                                            >  2:515561/1‑81 (MQ=255)                               cAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAAcc                                            >  2:515562/1‑81 (MQ=255)                                aGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAATCGTTGCACCAGACTTCGCCGCGACAAAAccc                                           >  2:515548/1‑81 (MQ=255)                                 ggcggcTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCcg                                          >  2:515521/1‑81 (MQ=255)                                  gcggcTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCcgc                                         <  2:515503/81‑1 (MQ=255)                                    ggcTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCgcgc                                       >  2:515549/1‑81 (MQ=255)                                         aaGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATc                                   <  2:515504/80‑1 (MQ=255)                                         aaGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCg                                  <  1:515505/81‑1 (MQ=255)                                            tGCGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAg                               <  1:515506/81‑1 (MQ=255)                                              cGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGa                              <  1:515507/80‑1 (MQ=255)                                              cGTAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAt                             <  2:515508/81‑1 (MQ=255)                                                tAAGTTCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAttt                           <  1:515509/81‑1 (MQ=255)                                                    ttCCGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTtcat                       <  2:515510/81‑1 (MQ=255)                                                       cGCCCCTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAg                    >  2:515565/1‑81 (MQ=255)                                                         ccccTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCg                  <  1:515511/81‑1 (MQ=255)                                                            cTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGc               <  2:515513/81‑1 (MQ=255)                                                            cTTCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGc               <  1:515512/81‑1 (MQ=255)                                                              tCAATACGCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCt             >  1:515571/1‑81 (MQ=255)                                                                     gCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtgg      >  2:515555/1‑81 (MQ=255)                                                                     gCACAGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtgg      >  2:515569/1‑81 (MQ=255)                                                                         aGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAt  <  2:515515/81‑1 (MQ=255)                                                                         aGGAGTAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAt  <  1:515514/81‑1 (MQ=255)                                                                             |                                                                             CGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAT  >  NC_000913/2975541‑2975693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑