Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,381	G→A	V226M (GTG→ATG)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,381	0	G	A	100.0%	99.1 / NA	29	V226M (GTG→ATG)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/18);  total (11/18)

GAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGT  >  NC_000913/353305‑353454                                                                             |                                                                          gAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGatggc                                                                       >  1:68638/1‑81 (MQ=255)  aaaaTGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGatggcg                                                                      <  1:68574/81‑1 (MQ=255)        gcgcgcGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCt                                                                 <  1:68575/80‑1 (MQ=255)          gcgcgTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTgg                                                               >  1:68628/1‑80 (MQ=255)            gcgTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAAt                                                            <  1:68576/81‑1 (MQ=255)            gcgTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAAt                                                            >  1:68632/1‑81 (MQ=255)              gTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATcc                                                          <  1:68577/81‑1 (MQ=255)                 aGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAAc                                                       >  2:68635/1‑81 (MQ=255)                     ggcgggATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGaaa                                                   >  1:68631/1‑81 (MQ=255)                      gcgggATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAAc                                                  <  2:68579/81‑1 (MQ=255)                      gcgggATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAAc                                                  <  2:68578/81‑1 (MQ=255)                        gggATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAAcct                                                <  1:68580/81‑1 (MQ=255)                           aTGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAAcctcct                                             >  2:68634/1‑81 (MQ=255)                            tGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTg                                            <  1:68581/81‑1 (MQ=255)                                  tttCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATtct                                      >  1:68637/1‑81 (MQ=255)                                      gcgTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTa                                   <  1:68582/80‑1 (MQ=255)                                      gcgTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTa                                   >  1:68649/1‑80 (MQ=255)                                         tcgtTACGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACt                               <  1:68583/81‑1 (MQ=255)                                                      aaCACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGAcc                   <  1:68584/80‑1 (MQ=255)                                                        cacaTCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCgg                 >  1:68647/1‑80 (MQ=255)                                                        cacaTCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGc                <  1:68586/81‑1 (MQ=255)                                                        cacaTCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGc                <  2:68587/81‑1 (MQ=255)                                                        cacaTCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGc                <  1:68585/81‑1 (MQ=255)                                                         acaTCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCa               <  2:68588/81‑1 (MQ=255)                                                              ggCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAAcc           >  2:68645/1‑80 (MQ=255)                                                                cgcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTaa        <  2:68589/81‑1 (MQ=255)                                                                cgcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTaa        <  1:68591/81‑1 (MQ=255)                                                                 gcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTa         >  2:68646/1‑79 (MQ=255)                                                                    cgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGc    <  1:68592/81‑1 (MQ=255)                                                                     gggTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCg   >  1:68642/1‑81 (MQ=255)                                                                      ggTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGt  <  1:68593/81‑1 (MQ=255)                                                                             |                                                                          GAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGT  >  NC_000913/353305‑353454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑