Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,008,057	G→A	G183D (GGC→GAC)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,008,057	0	G	A	100.0%	153.9 / NA	44	G183D (GGC→GAC)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

CGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCC  >  NC_000913/3007980‑3008134                                                                              |                                                                              cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTg                                                                            >  2:520346/1‑81 (MQ=255)  ggTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACt                                                                             <  2:520305/79‑1 (MQ=255)  ggTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTgg                                                                           >  2:520359/1‑81 (MQ=255)    tGCCGAGTGCAACGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGa                                                                         <  2:520306/81‑1 (MQ=255)     gCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTggg                                                                          >  1:520348/1‑79 (MQ=255)           tGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTa                                                                  <  2:520307/81‑1 (MQ=255)            gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTa                                                                  >  2:520356/1‑80 (MQ=255)            gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTa                                                                  >  1:520385/1‑80 (MQ=255)              aTCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAg                                                               <  2:520308/81‑1 (MQ=255)                cGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGa                                                             >  2:520366/1‑81 (MQ=255)                    aCGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACg                                                         >  2:520353/1‑81 (MQ=255)                     cGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACgg                                                        >  2:520370/1‑81 (MQ=255)                      ggATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGc                                                       <  1:520309/81‑1 (MQ=255)                       gATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCa                                                      >  2:520354/1‑81 (MQ=255)                             aaCTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGa                                                 <  2:520310/80‑1 (MQ=255)                               cTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAgg                                              <  2:520311/81‑1 (MQ=255)                                tATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGt                                             >  2:520372/1‑81 (MQ=255)                                 aTGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGt                                             >  1:520360/1‑80 (MQ=255)                                  tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGt                                             <  1:520313/79‑1 (MQ=255)                                  tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTa                                           <  2:520312/81‑1 (MQ=255)                                    aCGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTac                                          <  2:520314/80‑1 (MQ=255)                                     cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTacac                                        <  1:520315/81‑1 (MQ=255)                                      gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACAcc                                       <  2:520316/81‑1 (MQ=255)                                       aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACAcc                                       >  2:520387/1‑80 (MQ=255)                                       aTACCGTTCGCCTGACCATGCAACACCCGCAGCAGCACGACCGGGAAGTGGTCCAGCACACGGCCTGGGAAGGTCACAcc                                       >  2:520378/1‑80 (MQ=255)                                           cGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAAt                                  >  2:520355/1‑81 (MQ=255)                                             ttCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATcc                                >  2:520362/1‑81 (MQ=255)                                                gCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCaa                             >  1:520380/1‑81 (MQ=255)                                                    gACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTg                         >  2:520375/1‑81 (MQ=255)                                                    gACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTg                         >  2:520368/1‑81 (MQ=255)                                                        aTGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc                     <  2:520317/81‑1 (MQ=255)                                                         tGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa                    >  2:520373/1‑81 (MQ=255)                                                         tGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa                    >  2:520386/1‑81 (MQ=255)                                                           cAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCAt                   <  2:520318/80‑1 (MQ=255)                                                            aaCACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc                 <  1:520319/81‑1 (MQ=255)                                                               acGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg              <  2:520321/81‑1 (MQ=255)                                                               acGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg              <  1:520320/81‑1 (MQ=255)                                                                  cgcAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGc            >  2:520383/1‑80 (MQ=255)                                                                  cgcAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCt           <  2:520322/81‑1 (MQ=255)                                                                    cagcagcaCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTAc         <  1:520323/81‑1 (MQ=255)                                                                       cagcaCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCa      <  1:520324/81‑1 (MQ=255)                                                                         gcaCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAc    >  1:520371/1‑81 (MQ=255)                                                                          caCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAcc   >  2:520393/1‑81 (MQ=255)                                                                           aCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc  >  2:520374/1‑81 (MQ=255)                                                                              |                                                                              CGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCC  >  NC_000913/3007980‑3008134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑