Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,008,343	T→C	V278V (GTT→GTC)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,008,343	0	T	C	100.0%	142.9 / NA	40	V278V (GTT→GTC)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

GCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTA  >  NC_000913/3008268‑3008415                                                                            |                                                                         gCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTcggcgg                                                                     >  1:520436/1‑81 (MQ=255)  cacaGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCg                                                                      <  1:520381/79‑1 (MQ=255)  cacaGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGt                                                                    >  2:520443/1‑81 (MQ=255)       cATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGatat                                                               <  2:520382/81‑1 (MQ=255)       cATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGatat                                                               >  1:520448/1‑81 (MQ=255)         ttattGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATgg                                                             <  1:520383/81‑1 (MQ=255)          tattGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGc                                                            >  1:520440/1‑81 (MQ=255)           attGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGc                                                            >  2:520428/1‑80 (MQ=255)            ttgttgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGcc                                                           <  2:520385/80‑1 (MQ=255)            ttgttgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGcc                                                           <  1:520384/80‑1 (MQ=255)                tgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcat                                                      >  2:520417/1‑81 (MQ=255)                 gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcat                                                      <  1:520386/80‑1 (MQ=255)                  aaCCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcatca                                                    >  2:520437/1‑81 (MQ=255)                    ccTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATg                                                  <  1:520387/81‑1 (MQ=255)                       gACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATgg                                                 >  2:520447/1‑79 (MQ=255)                       gACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATgg                                                 >  2:520429/1‑79 (MQ=255)                         cAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAgg                                             >  1:520433/1‑81 (MQ=255)                          aaaGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggc                                            >  1:520445/1‑81 (MQ=255)                          aaaGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggc                                            >  2:520449/1‑81 (MQ=255)                           aaGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcc                                           >  2:520430/1‑81 (MQ=255)                             gCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctg                                         <  1:520388/81‑1 (MQ=255)                              cTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctgg                                        >  2:520427/1‑81 (MQ=255)                              cTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctgg                                        >  1:520455/1‑81 (MQ=255)                                gACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctggcc                                      >  1:520441/1‑81 (MQ=255)                                 tcTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctggcct                                     <  2:520389/80‑1 (MQ=255)                                       tttATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCg                               <  1:520390/81‑1 (MQ=255)                                          aTCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAAc                            >  1:520432/1‑81 (MQ=255)                                                 cGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAAcc                      <  2:520391/80‑1 (MQ=255)                                                  ggCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCg                    >  2:520451/1‑81 (MQ=255)                                                   gCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgc                   <  2:520392/81‑1 (MQ=255)                                                    cGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgct                  >  1:520438/1‑81 (MQ=255)                                                     gTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctg                 <  1:520393/81‑1 (MQ=255)                                                      tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg                <  2:520394/81‑1 (MQ=255)                                                      tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg                <  1:520397/81‑1 (MQ=255)                                                      tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg                <  1:520396/81‑1 (MQ=255)                                                      tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg                <  1:520395/81‑1 (MQ=255)                                                         aaGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgggct             >  1:520442/1‑81 (MQ=255)                                                           ggCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgggctgg           >  1:520454/1‑81 (MQ=255)                                                                 aTCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATc     >  2:520444/1‑81 (MQ=255)                                                                    gTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCt   <  2:520398/80‑1 (MQ=255)                                                                    gTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTa  <  1:520399/81‑1 (MQ=255)                                                                            |                                                                         GCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTA  >  NC_000913/3008268‑3008415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑