Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,863	C→A	I268I (ATC→ATA)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,863	0	C	A	100.0%	122.5 / NA	35	I268I (ATC→ATA)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/16);  total (19/16)

CTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACT  >  NC_000913/3016789‑3016940                                                                           |                                                                              cTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGAt                                                                         >  1:522008/1‑81 (MQ=255)     aagaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCt                                                                     <  1:521957/81‑1 (MQ=255)      agaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTg                                                                    <  1:521958/81‑1 (MQ=255)       gaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGc                                                                   <  2:521959/81‑1 (MQ=255)         aaaaGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCgg                                                                 >  2:522023/1‑81 (MQ=255)          aaaGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGt                                                                >  2:521997/1‑81 (MQ=255)            aGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGttt                                                              >  2:522006/1‑81 (MQ=255)             gggCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTc                                                             >  2:521999/1‑81 (MQ=255)                cTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGt                                                          <  1:521960/81‑1 (MQ=255)                 tCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGt                                                          >  2:701367/1‑80 (MQ=255)                   aaCACCTTTGTGAAACTTAACCTGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTa                                                        >  1:522005/1‑80 (MQ=255)                   aaCACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTa                                                        <  1:521961/80‑1 (MQ=255)                       ccTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAg                                                   >  2:522015/1‑81 (MQ=255)                       ccTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAg                                                   >  1:522019/1‑81 (MQ=255)                              gAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTaa                                             >  2:522016/1‑80 (MQ=255)                                    tAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaagaa                                        >  2:522010/1‑79 (MQ=255)                                     aaCCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaagaag                                       <  2:521962/79‑1 (MQ=255)                                     aaCCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAagaaga                                      <  1:521963/80‑1 (MQ=255)                                     aaCCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAagag                                     >  1:522007/1‑81 (MQ=255)                                       cccGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTc                                   <  1:521964/81‑1 (MQ=255)                                           aCCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCAttt                               <  1:521965/81‑1 (MQ=255)                                           aCCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCAttt                               >  1:522020/1‑81 (MQ=255)                                            ccTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCAttt                               >  1:522021/1‑80 (MQ=255)                                            ccTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTg                              <  2:521966/81‑1 (MQ=255)                                                 cTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCAc                         <  1:521967/81‑1 (MQ=255)                                                   ggggTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGa                       >  1:522017/1‑81 (MQ=255)                                                      gTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCt                    <  1:521968/81‑1 (MQ=255)                                                          gcgcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAg                >  2:522022/1‑81 (MQ=255)                                                            gcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt              <  2:521969/81‑1 (MQ=255)                                                            gcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt              >  2:521993/1‑81 (MQ=255)                                                                   cGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc         >  2:522011/1‑79 (MQ=255)                                                                   cGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCa        <  1:521971/80‑1 (MQ=255)                                                                   cGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCa        <  2:521970/80‑1 (MQ=255)                                                                     tGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc     <  1:521972/81‑1 (MQ=255)                                                                        gaTACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACt  >  2:522013/1‑81 (MQ=255)                                                                           |                                                                              CTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACT  >  NC_000913/3016789‑3016940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑