| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,017,496 | A→T | E479D (GAA→GAT) | ygfK → | putative oxidoreductase, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,017,496 | 0 | A | T | 97.6% | 143.9 / ‑3.9 | 42 | E479D (GAA→GAT) | ygfK | putative oxidoreductase, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (11/30); total (11/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC > NC_000913/3017420‑3017572 | aCTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCAcc < 2:522107/81‑1 (MQ=255) cTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGc < 2:522108/79‑1 (MQ=255) cTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCt < 2:522109/80‑1 (MQ=255) tACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTAt > 2:522160/1‑81 (MQ=255) gTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGcc < 1:522110/81‑1 (MQ=255) ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg < 1:522111/81‑1 (MQ=255) ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg < 2:522112/81‑1 (MQ=255) cccccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAc < 1:522113/80‑1 (MQ=255) ccccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAcgc < 1:522114/81‑1 (MQ=255) cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt < 1:522115/81‑1 (MQ=255) cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt < 1:522116/81‑1 (MQ=255) tgtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTgg < 2:522118/80‑1 (MQ=255) tgtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTgg < 1:522117/80‑1 (MQ=255) gtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGaa > 2:522165/1‑81 (MQ=255) ctgcctgcGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTc < 2:522119/80‑1 (MQ=255) gcgcTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTAc > 2:522168/1‑80 (MQ=255) cgcTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTAcc < 1:522120/80‑1 (MQ=255) cgcTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACca < 1:522121/81‑1 (MQ=255) aTCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacg > 1:522161/1‑80 (MQ=255) agcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGACGACGCGCTGGAACTCATCTACcaacgcaacg < 2:522122/81‑1 (MQ=255) gcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCaacgcaacgc > 2:522172/1‑81 (MQ=255) gcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCaacgcaacgc > 2:522171/1‑81 (MQ=255) gATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTg > 1:522169/1‑81 (MQ=255) atatTCAGGAATAGATCCGTCTGATTGGCGATAAGAGCTATGACGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGc < 1:522123/81‑1 (MQ=255) ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt > 1:522183/1‑81 (MQ=255) ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt > 2:522177/1‑81 (MQ=255) ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt < 1:522124/81‑1 (MQ=255) ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt < 1:522125/81‑1 (MQ=255) aaTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTa > 1:522174/1‑81 (MQ=255) aTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAc < 1:522126/81‑1 (MQ=255) tACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAcc < 2:522127/81‑1 (MQ=255) tACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAcc < 2:522128/81‑1 (MQ=255) cATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCg < 2:522129/80‑1 (MQ=255) aTCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGt < 2:522130/81‑1 (MQ=255) tCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTc < 1:522131/81‑1 (MQ=255) cGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCat < 2:522132/81‑1 (MQ=255) cGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCat < 1:522133/81‑1 (MQ=255) gCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTg > 2:522181/1‑81 (MQ=255) cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAttt < 1:522134/79‑1 (MQ=255) cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGc < 1:522135/81‑1 (MQ=255) ggCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCg < 2:522136/79‑1 (MQ=255) gCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATc < 2:522137/81‑1 (MQ=255) | ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC > NC_000913/3017420‑3017572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |