Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,034,269	T→C	A302A (GCA→GCG)	lysS ←	lysine‑‑tRNA ligase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,034,269	0	T	C	100.0%	135.1 / NA	38	A302A (GCA→GCG)	lysS	lysine‑‑tRNA ligase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/24);  total (14/24)

GGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCA  >  NC_000913/3034191‑3034345                                                                               |                                                                             ggTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTgcgc                                                                            >  2:525595/1‑81 (MQ=255)  gTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTgcgc                                                                            >  1:525596/1‑80 (MQ=255)     aGTTTTTCGAACGGTTTACCGAAGTCCAGCATCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCag                                                                         <  2:525550/80‑1 (MQ=255)       tttttCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGt                                                                      <  2:525551/81‑1 (MQ=255)          ttCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACg                                                                   >  2:525598/1‑81 (MQ=255)             gAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGaa                                                                <  2:525552/81‑1 (MQ=255)                cGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAg                                                             <  2:525553/81‑1 (MQ=255)                     tACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGAtt                                                        >  1:525591/1‑81 (MQ=255)                     tACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGAt                                                         <  1:525554/80‑1 (MQ=255)                      aCCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc                                                       >  2:525636/1‑81 (MQ=255)                       ccGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc                                                       <  1:525555/80‑1 (MQ=255)                       ccGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc                                                       <  1:525556/80‑1 (MQ=255)                       ccGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTc                                                       <  1:525557/80‑1 (MQ=255)                          aaGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGt                                                    >  2:525576/1‑80 (MQ=255)                          aaGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTc                                                   <  1:525558/81‑1 (MQ=255)                               cAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTc                                              <  2:525559/81‑1 (MQ=255)                               cAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTc                                              <  1:525560/81‑1 (MQ=255)                                aGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCg                                             <  2:525561/81‑1 (MQ=255)                                  cGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGAt                                           >  1:525614/1‑81 (MQ=255)                                      aCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAg                                        <  2:525562/80‑1 (MQ=255)                                         tCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                    <  2:525563/81‑1 (MQ=255)                                         tCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                    <  1:525564/81‑1 (MQ=255)                                          cGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt                                   >  1:525602/1‑81 (MQ=255)                                               tAGGTCACTTCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa                              <  2:525565/81‑1 (MQ=255)                                                     acctCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc                         <  2:525566/77‑1 (MQ=255)                                                       ttCCGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGt                       >  2:525613/1‑80 (MQ=255)                                                         ccGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTaa                     <  1:525567/80‑1 (MQ=255)                                                         ccGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTaa                     <  1:525568/80‑1 (MQ=255)                                                          cGTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc                   >  1:525628/1‑81 (MQ=255)                                                           gTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGcc                  <  2:525570/81‑1 (MQ=255)                                                           gTCTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCG‑AAGCCa                 <  1:525569/81‑1 (MQ=255)                                                             cTTACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt                >  2:525606/1‑81 (MQ=255)                                                               tACCGAGAATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg               >  2:525604/1‑80 (MQ=255)                                                                   gagaATATCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag          >  1:525618/1‑81 (MQ=255)                                                                        tatCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCca     <  2:525571/81‑1 (MQ=255)                                                                        tatCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCca     <  2:525572/81‑1 (MQ=255)                                                                         atCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat    >  1:525600/1‑81 (MQ=255)                                                                          tCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat    <  2:525573/80‑1 (MQ=255)                                                                          tCCTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat    >  1:525610/1‑80 (MQ=255)                                                                           ccTGCGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca  >  2:525615/1‑81 (MQ=255)                                                                               |                                                                             GGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCA  >  NC_000913/3034191‑3034345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑