Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,867	G→A	G725G (GGC→GGT)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,867	0	G	A	100.0%	114.3 / NA	34	G725G (GGC→GGT)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/21);  total (13/21)

CGCGCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACGCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGT  >  NC_000913/3046789‑3046943                                                                               |                                                                             cgcgCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACAcc                                                                            >  1:528199/1‑81 (MQ=255)  gcgcCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCg                                                                           <  2:528137/81‑1 (MQ=255)     cccTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATc                                                                        <  1:528139/81‑1 (MQ=255)     cccTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATc                                                                        <  2:528138/81‑1 (MQ=255)         gACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTc                                                                    >  2:528140/1‑81 (MQ=255)          aCGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTc                                                                    <  2:528141/80‑1 (MQ=255)            gggTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCa                                                                 >  2:528180/1‑81 (MQ=255)            gggTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCa                                                                 >  1:528182/1‑81 (MQ=255)             ggTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCAt                                                                <  2:528142/81‑1 (MQ=255)               ttAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAc                                                              <  2:528143/81‑1 (MQ=255)                tAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAcc                                                             >  2:528185/1‑81 (MQ=255)                 aaCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAccc                                                            >  2:528190/1‑81 (MQ=255)                 aaCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAcc                                                             <  2:528144/80‑1 (MQ=255)                  aCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAccc                                                            <  2:528145/80‑1 (MQ=255)                  aCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCg                                                           >  2:528183/1‑81 (MQ=255)                           cGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccacc                                                    <  2:528146/79‑1 (MQ=255)                            gATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccaccacc                                                 >  1:528208/1‑81 (MQ=255)                            gATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccaccacc                                                 <  2:528147/81‑1 (MQ=255)                               ttGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCAccgcc                                              >  1:528186/1‑81 (MQ=255)                                tGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCAccgcc                                              <  1:528148/80‑1 (MQ=255)                                       cGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGaa                                      <  1:528149/81‑1 (MQ=255)                                          tATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATg                                    >  1:528193/1‑80 (MQ=255)                                            tGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAg                                 <  2:528150/81‑1 (MQ=255)                                            tGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAg                                 <  2:528151/81‑1 (MQ=255)                                              aCCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGa                                <  1:528152/80‑1 (MQ=255)                                                ccGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAg                             >  2:528184/1‑81 (MQ=255)                                                   gTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGttt                          <  1:528153/81‑1 (MQ=255)                                                      cAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTAt                       <  2:528154/81‑1 (MQ=255)                                                            gTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGt                 <  2:528155/81‑1 (MQ=255)                                                             tGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGtt                <  2:528156/81‑1 (MQ=255)                                                             tGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGt                 >  1:528195/1‑80 (MQ=255)                                                                 aaaTGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAgg            <  1:528157/81‑1 (MQ=255)                                                                       gcTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGaa      <  1:528158/81‑1 (MQ=255)                                                                        cTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGaaa     >  1:528201/1‑81 (MQ=255)                                                                            cacaCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGt  <  2:528159/80‑1 (MQ=255)                                                                               |                                                                             CGCGCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACGCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGT  >  NC_000913/3046789‑3046943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑