Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,237	T→C	I316V (ATA→GTA)	yggF ←	fructose 1,6‑bisphosphatase YggF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,237	0	T	C	100.0%	91.6 / NA	26	I316V (ATA→GTA)	yggF	fructose 1,6‑bisphosphatase YggF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

GCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTT  >  NC_000913/3075162‑3075312                                                                            |                                                                            gCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtattacatta                                                                         >  1:534032/1‑80 (MQ=255) gCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtattacatta                                                                         <  1:533982/80‑1 (MQ=255)  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTAttacattaca                                                                       >  2:534028/1‑81 (MQ=255)     aaGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTacac                                                                      <  1:533984/79‑1 (MQ=255)     aaGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTacac                                                                      <  2:533983/79‑1 (MQ=255)     aaGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTacac                                                                      <  1:533985/79‑1 (MQ=255)      aGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGtt                                                                   <  1:533986/81‑1 (MQ=255)        cacaTCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTg                                                                 <  1:533987/81‑1 (MQ=255)          catcatCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGACCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGt                                                               >  1:534056/1‑81 (MQ=255)           atcatcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTc                                                              >  1:534043/1‑81 (MQ=255)            tcatcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTcc                                                             <  1:533988/81‑1 (MQ=255)              atcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCcgc                                                           >  2:534062/1‑81 (MQ=255)                  tctGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGcgccg                                                       >  2:534030/1‑81 (MQ=255)                       cAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGAt                                                  >  2:534039/1‑81 (MQ=255)                         gCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGAt                                                  >  2:534031/1‑79 (MQ=255)                          cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAg                                               >  2:534024/1‑81 (MQ=255)                            tCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTa                                             <  1:533989/81‑1 (MQ=255)                              gCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAAt                                           >  1:534050/1‑81 (MQ=255)                                  ttGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCt                                       <  2:533990/81‑1 (MQ=255)                                          ccGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTc                                  <  1:533991/78‑1 (MQ=255)                                          ccGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCg                                >  1:534045/1‑80 (MQ=255)                                           cGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCa                              <  2:533992/81‑1 (MQ=255)                                              tAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAccc                           >  1:534034/1‑81 (MQ=255)                                                gATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCa                         >  1:534052/1‑81 (MQ=255)                                                  tCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCAtt                       >  2:534066/1‑81 (MQ=255)                                                    aCCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTc                      <  1:533993/80‑1 (MQ=255)                                                              cAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGCCTGCGTCCGCACCCCATTCGCCGTCTGtt            <  1:533994/80‑1 (MQ=255)                                                                  gAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATa       >  1:534040/1‑81 (MQ=255)                                                                      cTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGATGGATACCGt   >  1:534048/1‑81 (MQ=255)                                                                       tattacattaCACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt  >  1:534055/1‑81 (MQ=255)                                                                        attacattacACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt  >  2:534057/1‑80 (MQ=255)                                                                            |                                                                            GCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTT  >  NC_000913/3075162‑3075312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑